Preparation of pH-sensitive liposomes retaining SOD mimic and their anticancer effect

Kazue Hiraka, Mayuko Kanehisa, Miho Tamai, Shoichiro Asayama, Shoji Nagaoka, Kenichi Oyaizu, Makoto Yuasa, Hiroyoshi Kawakami

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We prepared an anticancer drug based on a pH-sensitive liposome retaining Fe-porphyrin as an SOD mimic. The liposomes contained cationic/anionic lipid combinations and were composed of Fe-porphyrin, 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine, dimethylditetradecylammonium bromide, sodium oleate, and Tween-80. The Fe-porphyrin was released from the liposome at low pH, and the cytotoxicity for cancer cells by the liposome depended on the acidic environments of the endosomes in the cells. Furthermore, although the liposome exhibited an excellent anticancer effect on a gastric cancer cell line, the SOD activity of Fe-porphyrin was shown to have a significant influence on the cytotoxicity toward cancer cells. These findings suggest that the pH-sensitive liposome retaining the Fe-porphyrin as an SOD mimic promises to be a novel anticancer drug for endosomal escape.

Original languageEnglish
Pages (from-to)54-58
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume67
Issue number1
DOIs
Publication statusPublished - 2008 Nov 15
Externally publishedYes

Fingerprint

Liposomes
retaining
porphyrins
Porphyrins
preparation
cancer
Cells
Cytotoxicity
drugs
sodium bromides
cultured cells
Polysorbates
Endosomes
escape
lipids
Phosphatidylcholines
Pharmaceutical Preparations
Lipids
Stomach Neoplasms
Neoplasms

Keywords

  • Anticancer
  • Endosomal escape
  • Fe-porphyrin
  • pH-sensitive liposome
  • SOD mimic

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Preparation of pH-sensitive liposomes retaining SOD mimic and their anticancer effect. / Hiraka, Kazue; Kanehisa, Mayuko; Tamai, Miho; Asayama, Shoichiro; Nagaoka, Shoji; Oyaizu, Kenichi; Yuasa, Makoto; Kawakami, Hiroyoshi.

In: Colloids and Surfaces B: Biointerfaces, Vol. 67, No. 1, 15.11.2008, p. 54-58.

Research output: Contribution to journalArticle

Hiraka, K, Kanehisa, M, Tamai, M, Asayama, S, Nagaoka, S, Oyaizu, K, Yuasa, M & Kawakami, H 2008, 'Preparation of pH-sensitive liposomes retaining SOD mimic and their anticancer effect', Colloids and Surfaces B: Biointerfaces, vol. 67, no. 1, pp. 54-58. https://doi.org/10.1016/j.colsurfb.2008.07.014
Hiraka, Kazue ; Kanehisa, Mayuko ; Tamai, Miho ; Asayama, Shoichiro ; Nagaoka, Shoji ; Oyaizu, Kenichi ; Yuasa, Makoto ; Kawakami, Hiroyoshi. / Preparation of pH-sensitive liposomes retaining SOD mimic and their anticancer effect. In: Colloids and Surfaces B: Biointerfaces. 2008 ; Vol. 67, No. 1. pp. 54-58.
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