Preparation of potentiometric acetylcholine sensor using electropolymerized insulating poly-1-aminopyrrole with polyion complex

T. Kuwahara, N. Yamakawa, Toshiyuki Momma, Tetsuya Osaka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A potentiometric acetylcholine microsensor was fabricated by use of a composite film consisting of polyion complex (PIC), which consisted with acetylcholine esterase (AChE), and the electrochemically inactive poly-1-aminopyrrole (iPAPy). The iPAPy-PIC (AChE) composite film on Pt electrode displayed potential response to acetylcholine concentration owing to pH change during the enzymatic reaction, where the product contains weak acid CH3COOH. The sensitivity of the iPAPy-PIC (AChE) electrode clearly demonstrated higher sensitivity with low detection limit (1 × 10-6 mol dm-3) than that of the iPPy-PIC (AChE) electrode of detection limit (1 × 10-5 mol dm-3). The mechanism of increasing the sensitivity of iPAPy-PIC (AChE) electrode was discussed the interaction of -NH2 of iPAPy with CH3 COOH, which would accelerate the dissociation of weak acid CH3COOH.

Original languageEnglish
Pages (from-to)598-602
Number of pages5
JournalElectrochemistry
Volume69
Issue number8
Publication statusPublished - 2001 Aug

Fingerprint

Potentiometric sensors
Acetylcholine
Esterases
Electrodes
Composite films
Microsensors
Acids

Keywords

  • Electropolymerization
  • Poly-1-aminopyrrole
  • Potentiometric acetylcholine sensor

ASJC Scopus subject areas

  • Electrochemistry

Cite this

Preparation of potentiometric acetylcholine sensor using electropolymerized insulating poly-1-aminopyrrole with polyion complex. / Kuwahara, T.; Yamakawa, N.; Momma, Toshiyuki; Osaka, Tetsuya.

In: Electrochemistry, Vol. 69, No. 8, 08.2001, p. 598-602.

Research output: Contribution to journalArticle

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AB - A potentiometric acetylcholine microsensor was fabricated by use of a composite film consisting of polyion complex (PIC), which consisted with acetylcholine esterase (AChE), and the electrochemically inactive poly-1-aminopyrrole (iPAPy). The iPAPy-PIC (AChE) composite film on Pt electrode displayed potential response to acetylcholine concentration owing to pH change during the enzymatic reaction, where the product contains weak acid CH3COOH. The sensitivity of the iPAPy-PIC (AChE) electrode clearly demonstrated higher sensitivity with low detection limit (1 × 10-6 mol dm-3) than that of the iPPy-PIC (AChE) electrode of detection limit (1 × 10-5 mol dm-3). The mechanism of increasing the sensitivity of iPAPy-PIC (AChE) electrode was discussed the interaction of -NH2 of iPAPy with CH3 COOH, which would accelerate the dissociation of weak acid CH3COOH.

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