Preparation of potentiometric acetylcholine sensor using electropolymerized insulating poly-1-aminopyrrole with polyion complex

T. Kuwahara; N. Yamakawa; T. Momma; T. Osaka

doi:10.5796/electrochemistry.69.598

Preparation of potentiometric acetylcholine sensor using electropolymerized insulating poly-1-aminopyrrole with polyion complex

T. Kuwahara, N. Yamakawa, T. Momma, T. Osaka

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A potentiometric acetylcholine microsensor was fabricated by use of a composite film consisting of polyion complex (PIC), which consisted with acetylcholine esterase (AChE), and the electrochemically inactive poly-1-aminopyrrole (iPAPy). The iPAPy-PIC (AChE) composite film on Pt electrode displayed potential response to acetylcholine concentration owing to pH change during the enzymatic reaction, where the product contains weak acid CH₃COOH. The sensitivity of the iPAPy-PIC (AChE) electrode clearly demonstrated higher sensitivity with low detection limit (1 × 10^-6 mol dm^-3) than that of the iPPy-PIC (AChE) electrode of detection limit (1 × 10^-5 mol dm^-3). The mechanism of increasing the sensitivity of iPAPy-PIC (AChE) electrode was discussed the interaction of -NH₂ of iPAPy with CH₃ COOH, which would accelerate the dissociation of weak acid CH₃COOH.

Original language	English
Pages (from-to)	598-602
Number of pages	5
Journal	Electrochemistry
Volume	69
Issue number	8
DOIs	https://doi.org/10.5796/electrochemistry.69.598
Publication status	Published - 2001 Aug

Keywords

Electropolymerization
Poly-1-aminopyrrole
Potentiometric acetylcholine sensor

ASJC Scopus subject areas

Electrochemistry

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title = "Preparation of potentiometric acetylcholine sensor using electropolymerized insulating poly-1-aminopyrrole with polyion complex",

abstract = "A potentiometric acetylcholine microsensor was fabricated by use of a composite film consisting of polyion complex (PIC), which consisted with acetylcholine esterase (AChE), and the electrochemically inactive poly-1-aminopyrrole (iPAPy). The iPAPy-PIC (AChE) composite film on Pt electrode displayed potential response to acetylcholine concentration owing to pH change during the enzymatic reaction, where the product contains weak acid CH3COOH. The sensitivity of the iPAPy-PIC (AChE) electrode clearly demonstrated higher sensitivity with low detection limit (1 × 10-6 mol dm-3) than that of the iPPy-PIC (AChE) electrode of detection limit (1 × 10-5 mol dm-3). The mechanism of increasing the sensitivity of iPAPy-PIC (AChE) electrode was discussed the interaction of -NH2 of iPAPy with CH3 COOH, which would accelerate the dissociation of weak acid CH3COOH.",

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author = "T. Kuwahara and N. Yamakawa and T. Momma and T. Osaka",

year = "2001",

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doi = "10.5796/electrochemistry.69.598",

language = "English",

volume = "69",

pages = "598--602",

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AU - Kuwahara, T.

AU - Yamakawa, N.

AU - Momma, T.

AU - Osaka, T.

PY - 2001/8

Y1 - 2001/8

N2 - A potentiometric acetylcholine microsensor was fabricated by use of a composite film consisting of polyion complex (PIC), which consisted with acetylcholine esterase (AChE), and the electrochemically inactive poly-1-aminopyrrole (iPAPy). The iPAPy-PIC (AChE) composite film on Pt electrode displayed potential response to acetylcholine concentration owing to pH change during the enzymatic reaction, where the product contains weak acid CH3COOH. The sensitivity of the iPAPy-PIC (AChE) electrode clearly demonstrated higher sensitivity with low detection limit (1 × 10-6 mol dm-3) than that of the iPPy-PIC (AChE) electrode of detection limit (1 × 10-5 mol dm-3). The mechanism of increasing the sensitivity of iPAPy-PIC (AChE) electrode was discussed the interaction of -NH2 of iPAPy with CH3 COOH, which would accelerate the dissociation of weak acid CH3COOH.

AB - A potentiometric acetylcholine microsensor was fabricated by use of a composite film consisting of polyion complex (PIC), which consisted with acetylcholine esterase (AChE), and the electrochemically inactive poly-1-aminopyrrole (iPAPy). The iPAPy-PIC (AChE) composite film on Pt electrode displayed potential response to acetylcholine concentration owing to pH change during the enzymatic reaction, where the product contains weak acid CH3COOH. The sensitivity of the iPAPy-PIC (AChE) electrode clearly demonstrated higher sensitivity with low detection limit (1 × 10-6 mol dm-3) than that of the iPPy-PIC (AChE) electrode of detection limit (1 × 10-5 mol dm-3). The mechanism of increasing the sensitivity of iPAPy-PIC (AChE) electrode was discussed the interaction of -NH2 of iPAPy with CH3 COOH, which would accelerate the dissociation of weak acid CH3COOH.

KW - Electropolymerization

KW - Poly-1-aminopyrrole

KW - Potentiometric acetylcholine sensor

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Preparation of potentiometric acetylcholine sensor using electropolymerized insulating poly-1-aminopyrrole with polyion complex

Abstract

Keywords

ASJC Scopus subject areas

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