Abstract
A potentiometric acetylcholine microsensor was fabricated by use of a composite film consisting of polyion complex (PIC), which consisted with acetylcholine esterase (AChE), and the electrochemically inactive poly-1-aminopyrrole (iPAPy). The iPAPy-PIC (AChE) composite film on Pt electrode displayed potential response to acetylcholine concentration owing to pH change during the enzymatic reaction, where the product contains weak acid CH3COOH. The sensitivity of the iPAPy-PIC (AChE) electrode clearly demonstrated higher sensitivity with low detection limit (1 × 10-6 mol dm-3) than that of the iPPy-PIC (AChE) electrode of detection limit (1 × 10-5 mol dm-3). The mechanism of increasing the sensitivity of iPAPy-PIC (AChE) electrode was discussed the interaction of -NH2 of iPAPy with CH3 COOH, which would accelerate the dissociation of weak acid CH3COOH.
| Original language | English |
|---|---|
| Pages (from-to) | 598-602 |
| Number of pages | 5 |
| Journal | Electrochemistry |
| Volume | 69 |
| Issue number | 8 |
| Publication status | Published - 2001 Aug |
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Keywords
- Electropolymerization
- Poly-1-aminopyrrole
- Potentiometric acetylcholine sensor
ASJC Scopus subject areas
- Electrochemistry
Cite this
Preparation of potentiometric acetylcholine sensor using electropolymerized insulating poly-1-aminopyrrole with polyion complex. / Kuwahara, T.; Yamakawa, N.; Momma, Toshiyuki; Osaka, Tetsuya.
In: Electrochemistry, Vol. 69, No. 8, 08.2001, p. 598-602.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preparation of potentiometric acetylcholine sensor using electropolymerized insulating poly-1-aminopyrrole with polyion complex
AU - Kuwahara, T.
AU - Yamakawa, N.
AU - Momma, Toshiyuki
AU - Osaka, Tetsuya
PY - 2001/8
Y1 - 2001/8
N2 - A potentiometric acetylcholine microsensor was fabricated by use of a composite film consisting of polyion complex (PIC), which consisted with acetylcholine esterase (AChE), and the electrochemically inactive poly-1-aminopyrrole (iPAPy). The iPAPy-PIC (AChE) composite film on Pt electrode displayed potential response to acetylcholine concentration owing to pH change during the enzymatic reaction, where the product contains weak acid CH3COOH. The sensitivity of the iPAPy-PIC (AChE) electrode clearly demonstrated higher sensitivity with low detection limit (1 × 10-6 mol dm-3) than that of the iPPy-PIC (AChE) electrode of detection limit (1 × 10-5 mol dm-3). The mechanism of increasing the sensitivity of iPAPy-PIC (AChE) electrode was discussed the interaction of -NH2 of iPAPy with CH3 COOH, which would accelerate the dissociation of weak acid CH3COOH.
AB - A potentiometric acetylcholine microsensor was fabricated by use of a composite film consisting of polyion complex (PIC), which consisted with acetylcholine esterase (AChE), and the electrochemically inactive poly-1-aminopyrrole (iPAPy). The iPAPy-PIC (AChE) composite film on Pt electrode displayed potential response to acetylcholine concentration owing to pH change during the enzymatic reaction, where the product contains weak acid CH3COOH. The sensitivity of the iPAPy-PIC (AChE) electrode clearly demonstrated higher sensitivity with low detection limit (1 × 10-6 mol dm-3) than that of the iPPy-PIC (AChE) electrode of detection limit (1 × 10-5 mol dm-3). The mechanism of increasing the sensitivity of iPAPy-PIC (AChE) electrode was discussed the interaction of -NH2 of iPAPy with CH3 COOH, which would accelerate the dissociation of weak acid CH3COOH.
KW - Electropolymerization
KW - Poly-1-aminopyrrole
KW - Potentiometric acetylcholine sensor
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UR - http://www.scopus.com/inward/citedby.url?scp=0035416899&partnerID=8YFLogxK
M3 - Article
VL - 69
SP - 598
EP - 602
JO - Electrochemistry
JF - Electrochemistry
SN - 1344-3542
IS - 8
ER -