Preparation of single-cell arrays on caged cell-culturing substrates

Yukiko Kikuchi, Jun Nakanishi, Tohru Takarada, Hidekazu Nakayama, Kazuo Yamaguchi, Yasuhiko Yoshida, Mizuo Maeda

Research output: Contribution to conferencePaper

Abstract

Caged cell-culturing substrate is composed of a glass substrate coated with an alkylsiloxane monolayer having a photocleavable 2-nitrobenzyl group. In the present study, we examined the effect of terminal functional groups on the efficiencies of photoactivation for cell adhesion. Among four tested substrates terminating with different functional groups, the photoactivation of cell adhesion was the most effective on that terminating with an amino group. We succeeded in preparing single-cell arrays on this substrate without using fibronectin.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

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Keywords

  • Caged compound
  • Cell adhesion
  • Cell array
  • Functional group
  • Self-assembled monolayer

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kikuchi, Y., Nakanishi, J., Takarada, T., Nakayama, H., Yamaguchi, K., Yoshida, Y., & Maeda, M. (2005). Preparation of single-cell arrays on caged cell-culturing substrates. Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.