Preparation of single-cell arrays on caged cell-culturing substrates

Yukiko Kikuchi; Jun Nakanishi; Tohru Takarada; Hidekazu Nakayama; Kazuo Yamaguchi; Yasuhiko Yoshida; Mizuo Maeda

Preparation of single-cell arrays on caged cell-culturing substrates

Yukiko Kikuchi^*, Jun Nakanishi, Tohru Takarada, Hidekazu Nakayama, Kazuo Yamaguchi, Yasuhiko Yoshida, Mizuo Maeda

^*Corresponding author for this work

Graduate School of Advanced Science and Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

Caged cell-culturing substrate is composed of a glass substrate coated with an alkylsiloxane monolayer having a photocleavable 2-nitrobenzyl group. In the present study, we examined the effect of terminal functional groups on the efficiencies of photoactivation for cell adhesion. Among four tested substrates terminating with different functional groups, the photoactivation of cell adhesion was the most effective on that terminating with an amino group. We succeeded in preparing single-cell arrays on this substrate without using fibronectin.

Original language	English
Pages	4998
Number of pages	1
Publication status	Published - 2005
Event	54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: 2005 Sept 20 → 2005 Sept 22

Other

Other	54th SPSJ Symposium on Macromolecules
Country/Territory	Japan
City	Yamagata
Period	05/9/20 → 05/9/22

Keywords

Caged compound
Cell adhesion
Cell array
Functional group
Self-assembled monolayer

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "Preparation of single-cell arrays on caged cell-culturing substrates",

abstract = "Caged cell-culturing substrate is composed of a glass substrate coated with an alkylsiloxane monolayer having a photocleavable 2-nitrobenzyl group. In the present study, we examined the effect of terminal functional groups on the efficiencies of photoactivation for cell adhesion. Among four tested substrates terminating with different functional groups, the photoactivation of cell adhesion was the most effective on that terminating with an amino group. We succeeded in preparing single-cell arrays on this substrate without using fibronectin.",

keywords = "Caged compound, Cell adhesion, Cell array, Functional group, Self-assembled monolayer",

author = "Yukiko Kikuchi and Jun Nakanishi and Tohru Takarada and Hidekazu Nakayama and Kazuo Yamaguchi and Yasuhiko Yoshida and Mizuo Maeda",

year = "2005",

language = "English",

pages = "4998",

}

TY - CONF

T1 - Preparation of single-cell arrays on caged cell-culturing substrates

AU - Kikuchi, Yukiko

AU - Nakanishi, Jun

AU - Takarada, Tohru

AU - Nakayama, Hidekazu

AU - Yamaguchi, Kazuo

AU - Yoshida, Yasuhiko

AU - Maeda, Mizuo

PY - 2005

Y1 - 2005

N2 - Caged cell-culturing substrate is composed of a glass substrate coated with an alkylsiloxane monolayer having a photocleavable 2-nitrobenzyl group. In the present study, we examined the effect of terminal functional groups on the efficiencies of photoactivation for cell adhesion. Among four tested substrates terminating with different functional groups, the photoactivation of cell adhesion was the most effective on that terminating with an amino group. We succeeded in preparing single-cell arrays on this substrate without using fibronectin.

AB - Caged cell-culturing substrate is composed of a glass substrate coated with an alkylsiloxane monolayer having a photocleavable 2-nitrobenzyl group. In the present study, we examined the effect of terminal functional groups on the efficiencies of photoactivation for cell adhesion. Among four tested substrates terminating with different functional groups, the photoactivation of cell adhesion was the most effective on that terminating with an amino group. We succeeded in preparing single-cell arrays on this substrate without using fibronectin.

KW - Caged compound

KW - Cell adhesion

KW - Cell array

KW - Functional group

KW - Self-assembled monolayer

UR - http://www.scopus.com/inward/record.url?scp=33645641439&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645641439&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645641439

SP - 4998

T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -

Preparation of single-cell arrays on caged cell-culturing substrates

Abstract

Other

Keywords

ASJC Scopus subject areas

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