TY - JOUR
T1 - Pretreatment of serum containing hemoglobin vesicles (oxygen carriers) to prevent their interference in laboratory tests
AU - Sakai, Hiromi
AU - Tomiyama, Kenichi
AU - Masada, Yohei
AU - Takeoka, Shinji
AU - Horinouchi, Hirohisa
AU - Kobayashi, Koichi
AU - Tsuchida, Eishun
N1 - Funding Information:
The authors gratefully acknowledge the help of Dr. K. Sou and Mr. Y. Naito at Waseda University in the preparation of HbV, and Mr. S. Tsuda and Mr. K. Koyama at BML Inc. for the discussion of experimental procedure. This work was supported in part by Health Sciences Research Grants (Research on Pharmaceutical and Medical Safety, Artificial Blood Project) of the Ministry of Health, Labour and Welfare, Japan, and Grants in Aid for Scientific Research from the Japan Society for the Promotion of Science (B12480268, B12558112).
PY - 2003
Y1 - 2003
N2 - Hemoglobin vesicles (HbV, diameter: 251 ± 81 nm) are artificial oxygen (O2) carriers encapsulating concentrated hemoglobin (Hb) solution with phospholipid bilayer membrane, and their O2 transporting ability in vivo has been extensively studied. It is important to clarify the interference of the HbV suspension in clinical laboratory tests performed on serum and to establish a pretreatment method to avoid such an interference. The HbV suspension, acellular Hb solution ([Hb] = 10 g/dl) or saline, was mixed with a pooled human serum at various ratios up to 50 vol% ([Hb] = 5 g/dl), and the magnitude of the interference effect of HbV and Hb on 30 analytes was studied. The mixture of the HbV suspension and serum was ultracentrifuged (50000 g, 20 min) to remove the HbV particles as precipitate, and the supernatant was analyzed and compared with the saline control group. The HbV particles were also removed by centrifugation (2700 g, 30 min) in the presence of dextran (Mw 200 kDa). The HbV suspension showed considerable interference effects in most analytes. The majority of these effects was more serious than those of the acellular Hb solution. These findings are thought to be due to the light absorption of Hb in HbV and/or the light scattering generated in the suspension that interferes with the colorimetric and turbidimetric measurements. The components of HbV may also interfere with the chemical reactions of the studied assays. However, removal of the HbV from the supernatant diminished the interference in most of the assays: this is an advantage of HbV in comparison with acellular chemically modified Hb solutions.
AB - Hemoglobin vesicles (HbV, diameter: 251 ± 81 nm) are artificial oxygen (O2) carriers encapsulating concentrated hemoglobin (Hb) solution with phospholipid bilayer membrane, and their O2 transporting ability in vivo has been extensively studied. It is important to clarify the interference of the HbV suspension in clinical laboratory tests performed on serum and to establish a pretreatment method to avoid such an interference. The HbV suspension, acellular Hb solution ([Hb] = 10 g/dl) or saline, was mixed with a pooled human serum at various ratios up to 50 vol% ([Hb] = 5 g/dl), and the magnitude of the interference effect of HbV and Hb on 30 analytes was studied. The mixture of the HbV suspension and serum was ultracentrifuged (50000 g, 20 min) to remove the HbV particles as precipitate, and the supernatant was analyzed and compared with the saline control group. The HbV particles were also removed by centrifugation (2700 g, 30 min) in the presence of dextran (Mw 200 kDa). The HbV suspension showed considerable interference effects in most analytes. The majority of these effects was more serious than those of the acellular Hb solution. These findings are thought to be due to the light absorption of Hb in HbV and/or the light scattering generated in the suspension that interferes with the colorimetric and turbidimetric measurements. The components of HbV may also interfere with the chemical reactions of the studied assays. However, removal of the HbV from the supernatant diminished the interference in most of the assays: this is an advantage of HbV in comparison with acellular chemically modified Hb solutions.
KW - Blood substitutes
KW - Hemoglobin
KW - Hemolysis
KW - Liposome
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U2 - 10.1515/CCLM.2003.036
DO - 10.1515/CCLM.2003.036
M3 - Article
C2 - 12667011
AN - SCOPUS:0037224899
VL - 41
SP - 222
EP - 231
JO - Zeitschrift fur klinische Chemie und klinische Biochemie
JF - Zeitschrift fur klinische Chemie und klinische Biochemie
SN - 1434-6621
IS - 2
ER -