Prevention of inhibitory effect of dorsal raphe nucleus lesions on ovulation and LH surge by 5-HT 2A/2C receptor agonists in female rats

Fumihiko Maekawa, Shinji Tsukahara, Hiroko Tsukamura, Kei Ichiro Maeda, Korehito Yamanouchi

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    In order to investigate the role of the dorsal raphe nucleus and the serotonergic system in the regulation of ovulation, the number of ova and plasma luteinizing hormone (LH) concentrations were measured in female rats after making lesions in this nucleus (DRL) and/or treatment with 5-hydroxytryptamine (5-HT) receptor agonists or antagonists. DRL or sham lesion was made on the afternoon of proestrous (12:00-14:00 h) under ether anesthesia and the number of ova in the oviduct was counted on the next estrous and diestrous morning. In some animals, blood samples were taken via the atrial cannula during the proestrous evening for the radioimmunoassay of LH. All intact control and sham-operated females ovulated and plasma LH increased between 19:00 and 21:00 h. In contrast, ovulation was seen in only 36% of DRL rats. LH surge did not occur in this group. However, 80% of DRL rats ovulated after treatment with (±)-1-(4-iodo-2,5-dimethoxyphenyl)-2-aminopropane hydrochloride [(±)-DOI; a 5-HT 2A/2C receptor agonist] at 15:00 h on proestrous day. LH surge was also observed in the DRL rats with (±)-DOI. On the other hand, only 8% of DRL rats ovulated after treatment with buspirone (5-HT 1A receptor agonist). Furthermore, when mianserin (5-HT 2A/2C receptor antagonist) was administered at 16:00 h on proestrous day, ovulation was not seen in all rats without DRL. These results suggest that the dorsal raphe nucleus plays an important role in induction of LH surge and ovulation and the 5-HT 2A/2C receptor system is involved in this mechanism. Copyright (C) 1999 Elsevier Science Ireland Ltd.

    Original languageEnglish
    Pages (from-to)291-298
    Number of pages8
    JournalNeuroscience Research
    Volume35
    Issue number4
    DOIs
    Publication statusPublished - 1999 Dec 30

    Fingerprint

    Luteinizing Hormone
    Ovulation
    Serotonin
    Ovum
    Mianserin
    Buspirone
    Serotonin Receptor Agonists
    Serotonin Antagonists
    Receptor, Serotonin, 5-HT1A
    Oviducts
    Serotonin Receptors
    Ether
    Radioimmunoassay
    Dorsal Raphe Nucleus
    Anesthesia

    Keywords

    • 5-HT 2A/2C receptors
    • Dorsal raphe nucleus
    • Lesion
    • Ovulation
    • Preovulatory LH surge

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Prevention of inhibitory effect of dorsal raphe nucleus lesions on ovulation and LH surge by 5-HT 2A/2C receptor agonists in female rats. / Maekawa, Fumihiko; Tsukahara, Shinji; Tsukamura, Hiroko; Maeda, Kei Ichiro; Yamanouchi, Korehito.

    In: Neuroscience Research, Vol. 35, No. 4, 30.12.1999, p. 291-298.

    Research output: Contribution to journalArticle

    Maekawa, Fumihiko ; Tsukahara, Shinji ; Tsukamura, Hiroko ; Maeda, Kei Ichiro ; Yamanouchi, Korehito. / Prevention of inhibitory effect of dorsal raphe nucleus lesions on ovulation and LH surge by 5-HT 2A/2C receptor agonists in female rats. In: Neuroscience Research. 1999 ; Vol. 35, No. 4. pp. 291-298.
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    abstract = "In order to investigate the role of the dorsal raphe nucleus and the serotonergic system in the regulation of ovulation, the number of ova and plasma luteinizing hormone (LH) concentrations were measured in female rats after making lesions in this nucleus (DRL) and/or treatment with 5-hydroxytryptamine (5-HT) receptor agonists or antagonists. DRL or sham lesion was made on the afternoon of proestrous (12:00-14:00 h) under ether anesthesia and the number of ova in the oviduct was counted on the next estrous and diestrous morning. In some animals, blood samples were taken via the atrial cannula during the proestrous evening for the radioimmunoassay of LH. All intact control and sham-operated females ovulated and plasma LH increased between 19:00 and 21:00 h. In contrast, ovulation was seen in only 36{\%} of DRL rats. LH surge did not occur in this group. However, 80{\%} of DRL rats ovulated after treatment with (±)-1-(4-iodo-2,5-dimethoxyphenyl)-2-aminopropane hydrochloride [(±)-DOI; a 5-HT 2A/2C receptor agonist] at 15:00 h on proestrous day. LH surge was also observed in the DRL rats with (±)-DOI. On the other hand, only 8{\%} of DRL rats ovulated after treatment with buspirone (5-HT 1A receptor agonist). Furthermore, when mianserin (5-HT 2A/2C receptor antagonist) was administered at 16:00 h on proestrous day, ovulation was not seen in all rats without DRL. These results suggest that the dorsal raphe nucleus plays an important role in induction of LH surge and ovulation and the 5-HT 2A/2C receptor system is involved in this mechanism. Copyright (C) 1999 Elsevier Science Ireland Ltd.",
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    AU - Maeda, Kei Ichiro

    AU - Yamanouchi, Korehito

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