Primary and higher order structures of nematode (Ascaris suum) mitochondrial tRNAs lacking either the T or D stem

Yoh Ichi Watanabe, Hiromichi Tsurui, Takuya Ueda, Rieko Furushima, Shinzaburo Takamiya, Kiyoshi Kita, Kazuya Nishikawa, Kimitsuna Watanabe

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Abstract

By fractionation using polyacrylamide gel electrophoresis and/or a preparative hybrid selection method employing solid-phase DNA probes, we prepared and characterized mitochondrial tRNAs from the body wall muscle of Ascaris suum, all of which are thought to lack either the T stem or the D stem from their gene sequences (Okimoto, R., and Wolstenholme, D. R. (1990) EMBO J. 10, 3405-3411). Some of the partially purified tRNAs were appreciably aminoacylated with an extract of A. suum mitochondria. The three species sequenced had CCA sequence at their 3'-ends, and tRNA(Met) had 5- formylcytidine at the anticodon first position, a new modified nucleoside found at the same position of bovine mitochondrial tRNA(Met) (Moriya, J., Yokogawa, T., Wakita, K., Ueda, T., Nishikawa, K., Crain, P. F., Hashizume, T., Pomerantz, S. C., McCloskey, J. A., Kawai, G., Hayashi, N., Yokoyama, S., and Watanabe, K. (1994) Biochemistry 33, 2234-2239). Enzymatic probing of these tRNAs supported the secondary structural model proposed by Okimoto and Wolstenholme in the reference cited above. Chemical probing of tRNA(Phe) demonstrated the existence of tertiary interactions between the (T arm- variable loop)-replacement loop and the D arm. The results suggest that these tertiary interactions enable the bizarre tRNAs of nematode mitochondria to maintain an L-shape-like structure in order to function in the nematode mitochondrial translation system.

Original languageEnglish
Pages (from-to)22902-22906
Number of pages5
JournalJournal of Biological Chemistry
Volume269
Issue number36
Publication statusPublished - 1994 Sep 9
Externally publishedYes

Fingerprint

Ascaris suum
Transfer RNA
RNA, Transfer, Met
Mitochondria
RNA, Transfer, Phe
Anticodon
Biochemistry
Structural Models
DNA Probes
Fractionation
Electrophoresis
Nucleosides
Muscle
Polyacrylamide Gel Electrophoresis
Genes
Muscles

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Watanabe, Y. I., Tsurui, H., Ueda, T., Furushima, R., Takamiya, S., Kita, K., ... Watanabe, K. (1994). Primary and higher order structures of nematode (Ascaris suum) mitochondrial tRNAs lacking either the T or D stem. Journal of Biological Chemistry, 269(36), 22902-22906.

Primary and higher order structures of nematode (Ascaris suum) mitochondrial tRNAs lacking either the T or D stem. / Watanabe, Yoh Ichi; Tsurui, Hiromichi; Ueda, Takuya; Furushima, Rieko; Takamiya, Shinzaburo; Kita, Kiyoshi; Nishikawa, Kazuya; Watanabe, Kimitsuna.

In: Journal of Biological Chemistry, Vol. 269, No. 36, 09.09.1994, p. 22902-22906.

Research output: Contribution to journalArticle

Watanabe, YI, Tsurui, H, Ueda, T, Furushima, R, Takamiya, S, Kita, K, Nishikawa, K & Watanabe, K 1994, 'Primary and higher order structures of nematode (Ascaris suum) mitochondrial tRNAs lacking either the T or D stem', Journal of Biological Chemistry, vol. 269, no. 36, pp. 22902-22906.
Watanabe, Yoh Ichi ; Tsurui, Hiromichi ; Ueda, Takuya ; Furushima, Rieko ; Takamiya, Shinzaburo ; Kita, Kiyoshi ; Nishikawa, Kazuya ; Watanabe, Kimitsuna. / Primary and higher order structures of nematode (Ascaris suum) mitochondrial tRNAs lacking either the T or D stem. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 36. pp. 22902-22906.
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