Principal component analysis of odor coding at the level of third-order olfactory neurons in Drosophila

Makoto Hiroi, Masamichi Ohkura, Junichi Nakai, Naoki Masuda, Koichi Hashimoto, Kiichi Inoue, André Fiala, Tetsuya Tabata

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Olfactory information in Drosophila is conveyed by projection neurons from olfactory sensory neurons to Kenyon cells (KCs) in the mushroom body (MB). A subset of KCs responds to a given odor molecule, and the combination of these KCs represents a part of the neuronal olfactory code. KCs are also thought to function as coincidence detectors for memory formation, associating odor information with a coincident punishment or reward stimulus. Associative conditioning has been shown to modify KC output. This plasticity occurs in the vertical lobes of MBs containing α/α' branches of KCs, which is shown by measuring the average Ca2+ levels in the branch of each lobe. We devised a method to quantitatively describe the population activity patterns recorded from axons of >1000 KCs at the α/α' branches using two-photon Ca2+ imaging. Principal component analysis of the population activity patterns clearly differentiated the responses to distinct odors.

Original languageEnglish
Pages (from-to)1070-1081
Number of pages12
JournalGenes to Cells
Volume18
Issue number12
DOIs
Publication statusPublished - 2013 Dec
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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