Printing support hydrogels for creating vascular-like structures in stacked cell sheets

Ryu ichiro Tanaka; Katsuhisa Sakaguchi; Shinjiro Umezu

doi:10.1007/s10015-020-00605-7

Printing support hydrogels for creating vascular-like structures in stacked cell sheets

Ryu ichiro Tanaka, Katsuhisa Sakaguchi, Shinjiro Umezu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Vascular structures are essential for the survival of thick artificial three-dimensional (3D) tissues. However, it is difficult to create high-cell-density artificial tissue with vascular structures of a few hundred micrometers in diameter. Bioprinting technology can create artificial 3D tissues with vascular structures of a few hundred micrometers in diameter, but the cell density of bio-printed artificial 3D tissues is low. On the other hand, cell sheet technology can create high-cell-density artificial 3D tissues by stacking, but it is not possible to set small vascular structures at any place. In this study, we successfully demonstrated high-cell-density artificial 3D tissues with vascular-like structures by stacking cell sheets combined with bioprinting technology.

Original language	English
Pages (from-to)	199-203
Number of pages	5
Journal	Artificial Life and Robotics
Volume	25
Issue number	2
DOIs	https://doi.org/10.1007/s10015-020-00605-7
Publication status	Published - 2020 May 1

Keywords

Bioprinting
Cell sheet
Support hydrogel
Vascular-like structure

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Artificial Intelligence

Access to Document

10.1007/s10015-020-00605-7

Cite this

@article{0314664b3d30431c9f2083554a4d411a,

title = "Printing support hydrogels for creating vascular-like structures in stacked cell sheets",

abstract = "Vascular structures are essential for the survival of thick artificial three-dimensional (3D) tissues. However, it is difficult to create high-cell-density artificial tissue with vascular structures of a few hundred micrometers in diameter. Bioprinting technology can create artificial 3D tissues with vascular structures of a few hundred micrometers in diameter, but the cell density of bio-printed artificial 3D tissues is low. On the other hand, cell sheet technology can create high-cell-density artificial 3D tissues by stacking, but it is not possible to set small vascular structures at any place. In this study, we successfully demonstrated high-cell-density artificial 3D tissues with vascular-like structures by stacking cell sheets combined with bioprinting technology.",

keywords = "Bioprinting, Cell sheet, Support hydrogel, Vascular-like structure",

author = "Tanaka, {Ryu ichiro} and Katsuhisa Sakaguchi and Shinjiro Umezu",

note = "Funding Information: This work was supported by Japan Society for the Promotion of Science (no. 18K18838) and Japan Science and Technology Agency (no. JPMJMI18CD). Publisher Copyright: {\textcopyright} 2020, International Society of Artificial Life and Robotics (ISAROB).",

year = "2020",

month = may,

day = "1",

doi = "10.1007/s10015-020-00605-7",

language = "English",

volume = "25",

pages = "199--203",

journal = "Artificial Life and Robotics",

issn = "1433-5298",

publisher = "Springer Japan",

number = "2",

}

TY - JOUR

T1 - Printing support hydrogels for creating vascular-like structures in stacked cell sheets

AU - Tanaka, Ryu ichiro

AU - Sakaguchi, Katsuhisa

AU - Umezu, Shinjiro

N1 - Funding Information: This work was supported by Japan Society for the Promotion of Science (no. 18K18838) and Japan Science and Technology Agency (no. JPMJMI18CD). Publisher Copyright: © 2020, International Society of Artificial Life and Robotics (ISAROB).

PY - 2020/5/1

Y1 - 2020/5/1

N2 - Vascular structures are essential for the survival of thick artificial three-dimensional (3D) tissues. However, it is difficult to create high-cell-density artificial tissue with vascular structures of a few hundred micrometers in diameter. Bioprinting technology can create artificial 3D tissues with vascular structures of a few hundred micrometers in diameter, but the cell density of bio-printed artificial 3D tissues is low. On the other hand, cell sheet technology can create high-cell-density artificial 3D tissues by stacking, but it is not possible to set small vascular structures at any place. In this study, we successfully demonstrated high-cell-density artificial 3D tissues with vascular-like structures by stacking cell sheets combined with bioprinting technology.

AB - Vascular structures are essential for the survival of thick artificial three-dimensional (3D) tissues. However, it is difficult to create high-cell-density artificial tissue with vascular structures of a few hundred micrometers in diameter. Bioprinting technology can create artificial 3D tissues with vascular structures of a few hundred micrometers in diameter, but the cell density of bio-printed artificial 3D tissues is low. On the other hand, cell sheet technology can create high-cell-density artificial 3D tissues by stacking, but it is not possible to set small vascular structures at any place. In this study, we successfully demonstrated high-cell-density artificial 3D tissues with vascular-like structures by stacking cell sheets combined with bioprinting technology.

KW - Bioprinting

KW - Cell sheet

KW - Support hydrogel

KW - Vascular-like structure

UR - http://www.scopus.com/inward/record.url?scp=85083434508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85083434508&partnerID=8YFLogxK

U2 - 10.1007/s10015-020-00605-7

DO - 10.1007/s10015-020-00605-7

M3 - Article

AN - SCOPUS:85083434508

VL - 25

SP - 199

EP - 203

JO - Artificial Life and Robotics

JF - Artificial Life and Robotics

SN - 1433-5298

IS - 2

ER -

Printing support hydrogels for creating vascular-like structures in stacked cell sheets

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this