Processing of multiple forms of preprosodefrin in the abdominal gland of the red-bellied newt Cynops pyrrhogaster

Regional and individual differences in preprosodefrin gene expression

Takeo Iwata, J. Michael Conlon, Tomoaki Nakada, Fumiyo Toyoda, Kazutoshi Yamamoto, Sakae Kikuyama

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Peptides derived from the post-translational processing of preprosodefrin were isolated from an extract of the abdominal glands of male red-bellied newts Cynops pyrrhogaster obtained 5 months prior to the onset of the breeding season. Structural characterization of the peptides showed that the pheromone sodefrin (SIPSKDALLK) is stored in a biologically inactive COOH-terminally extended form (SIPSKDALLKISA). It follows, therefore, that the activation of a protease that cleaves at a Lys-Ile bond to generate the active pheromone must occur by the time of onset of reproductive behavior. Additional peptides (representing preprosodefrin-(146-175)-peptide and preprosodefrin-(159-173)-peptide), that are derived from the precursor by cleavage at monobasic and dibasic processing sites, were also purified from the extract. The isolation of paralogs of these peptides, including an inactive COOH-terminally extended form of [Asn 10]sodefrin, provides evidence for the expression of multiple genes encoding preprosodefrin. PCR products derived from total RNAs from the abdominal gland of individual newts collected from three different regions of Japan were analyzed. The data confirm the existence of multiple genes encoding sodefrin and its variants whose expression varied according to the individuals and the regions. However, genes encoding sodefrin were found to be expressed in all the specimens sampled.

    Original languageEnglish
    Pages (from-to)1537-1543
    Number of pages7
    JournalPeptides
    Volume25
    Issue number9
    DOIs
    Publication statusPublished - 2004 Sep

    Fingerprint

    sodefrin
    Salamandridae
    Gene expression
    Individuality
    Gene Expression
    Peptides
    Gene encoding
    Processing
    Pheromones
    Reproductive Behavior
    Genes
    Breeding
    Japan
    Peptide Hydrolases
    Chemical activation
    RNA
    Polymerase Chain Reaction

    Keywords

    • Amphibian
    • Pheromone
    • Post-translational processing
    • Sodefrin

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Physiology
    • Cellular and Molecular Neuroscience

    Cite this

    Processing of multiple forms of preprosodefrin in the abdominal gland of the red-bellied newt Cynops pyrrhogaster : Regional and individual differences in preprosodefrin gene expression. / Iwata, Takeo; Conlon, J. Michael; Nakada, Tomoaki; Toyoda, Fumiyo; Yamamoto, Kazutoshi; Kikuyama, Sakae.

    In: Peptides, Vol. 25, No. 9, 09.2004, p. 1537-1543.

    Research output: Contribution to journalArticle

    Iwata, Takeo ; Conlon, J. Michael ; Nakada, Tomoaki ; Toyoda, Fumiyo ; Yamamoto, Kazutoshi ; Kikuyama, Sakae. / Processing of multiple forms of preprosodefrin in the abdominal gland of the red-bellied newt Cynops pyrrhogaster : Regional and individual differences in preprosodefrin gene expression. In: Peptides. 2004 ; Vol. 25, No. 9. pp. 1537-1543.
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