Production of β-fructofuranosidase showing fructose-transferring activity by Penicillium frequentans (P. glabrum)

Shoji Usami, Takashi Ishii, Kotaro Kirimura, Ken'ichi Uehara, Jiacong Chen

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Microorganisms producing enzymes showing fructose-transferring activities were screened from various samples such as soils, flowers, vegetables and fruits. One fungus isolated from azalea was found to produce β-fructofuranosidase, which shows fructose-transferring activity, when cultivated in media containing sucrose as a carbon source. From its growth and morphological characteristics, this strain was identified as Penicillium frequentans (P. glabrum). The strain utilized sucrose and raffinose but not inulin; sucrose was the best carbon source for the production of the enzyme. When cultivated for 4 d in a medium containing 15% (w/v) sucrose under optimal conditions, the fructose-transferring activity reached a maximum of 5.4 U/ml. Under these conditions, 95% of total enzyme activity was detected in the cell-free extract but not in the culture filtrate.

    Original languageEnglish
    Pages (from-to)303-305
    Number of pages3
    JournalJournal of Fermentation and Bioengineering
    Volume72
    Issue number4
    DOIs
    Publication statusPublished - 1991

    Fingerprint

    Fructose
    Penicillium
    Sugar (sucrose)
    Sucrose
    Enzymes
    Carbon
    Raffinose
    Inulin
    Vegetables
    Enzyme activity
    Fruits
    Cell Extracts
    Fungi
    Microorganisms
    Fruit
    Soil
    Soils
    Growth

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    Production of β-fructofuranosidase showing fructose-transferring activity by Penicillium frequentans (P. glabrum). / Usami, Shoji; Ishii, Takashi; Kirimura, Kotaro; Uehara, Ken'ichi; Chen, Jiacong.

    In: Journal of Fermentation and Bioengineering, Vol. 72, No. 4, 1991, p. 303-305.

    Research output: Contribution to journalArticle

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    AU - Uehara, Ken'ichi

    AU - Chen, Jiacong

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