Production of antioxidant vitamins, β-carotene, vitamin C, and vitamin E, by two-step culture of Euglena gracilis Z

Haruko Takeyama, Akihisa Kanamaru, Yuko Yoshino, Hiroyuki Kakuta, Yoshiya Kawamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Euglena gracilis Z is one of the few microorganisms which simultaneously produces antioxidant vitamins such as β-carotene and vitamins C and E. Photoheterotrophically cultured E. gracilis Z produced larger levels of biomass but with a lower content of antioxidant vitamins than photoautotrophically grown cultures. For efficient production of these vitamins, a two-step culture was performed. Cells were grown photoheterotrophically and then transferred to photoautotrophic conditions. When E. gracilis Z cells were grown in fed-batch culture under photoheterotrophic conditions, their density reached 19 g/L after 145 h. Subsequent transfer of these cells to photoautotrophic conditions increased vitamin content, enhancing the total vitamin yields, which were 71.0 mg/L of β-carotene, 30.1 mg/L of vitamin E, and 86.5 mg/L of vitamin C.

Original languageEnglish
Pages (from-to)185-190
Number of pages6
JournalBiotechnology and Bioengineering
Volume53
Issue number2
DOIs
Publication statusPublished - 1997 Jan 20
Externally publishedYes

Fingerprint

Euglena gracilis
Vitamins
Carotenoids
Antioxidants
Vitamin E
Ascorbic Acid
Batch Cell Culture Techniques
Microorganisms
Biomass

Keywords

  • β-carotene
  • antioxidant vitamins
  • Euglena gracilis
  • vitamin C
  • vitamin E

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Production of antioxidant vitamins, β-carotene, vitamin C, and vitamin E, by two-step culture of Euglena gracilis Z. / Takeyama, Haruko; Kanamaru, Akihisa; Yoshino, Yuko; Kakuta, Hiroyuki; Kawamura, Yoshiya; Matsunaga, Tadashi.

In: Biotechnology and Bioengineering, Vol. 53, No. 2, 20.01.1997, p. 185-190.

Research output: Contribution to journalArticle

Takeyama, Haruko ; Kanamaru, Akihisa ; Yoshino, Yuko ; Kakuta, Hiroyuki ; Kawamura, Yoshiya ; Matsunaga, Tadashi. / Production of antioxidant vitamins, β-carotene, vitamin C, and vitamin E, by two-step culture of Euglena gracilis Z. In: Biotechnology and Bioengineering. 1997 ; Vol. 53, No. 2. pp. 185-190.
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