Production of double-layered metal nanocups for artificial nanospace of biomolecular reaction

Hyonchol Kim, Masahito Hayashi, Hideyuki Terazono, Hiroyuki Takei, Kenji Yasuda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Nanocups (NCs), sub-micrometer semispherical bowls consisting of two different nanometer-thick metals on inner and outer layers, have been fabricated to mimic a localized nano-scale biochemical reaction environment for reactive biomolecules. Homogeneous polystyrene beads were used as a cast of the NCs, placed on a Si substrate, dried, and processed by oxygen plasma etching until the desired diameters and gaps among neighboring bead casts. For the fabrication of Au/Ni double-layered NCs, Au and Ni were sequentially deposited on upper halves of the bead surfaces by thermal evaporation with nanometer-order thickness control. The polystyrene casts were removed completely by UV-ozone oxidization reaction, and Au/Ni double-layered NCs were fabricated on a Si substrate. To orient the holes of the fabricated NCs to top for the substrate, poly(dimethylsiloxane) (PDMS) sol was dropped on the NCs placed on the Si substrate, hardened, and peeled off from the substrate, and then the NCs were placed on the PDMS surface with those holes turned-up. To examine the selective interaction of biomolecules on the inner layer of NCs as the artificial nanospace for biomolecular reactions, a thiolated target DNA was immobilized onto the inner layer of a Au/Ni NC as a model. The target DNA was labeled through hybridization reaction using small Au nanoparticles (NPs) on which a complementary probe DNA was immobilized. Both the surface-specific immobilization of the target DNA on the Au layer of the NC and the specific hybridization in NC nanospaces were confirmed by direct observations after those reactions using field emission scanning electron microscopy (FE-SEM), indicating that the inside of the fabricated NCs can be used as the artificial nanospace for studying localized biomolecular reactions.

Original languageEnglish
Article number06GJ03
JournalJapanese Journal of Applied Physics
Volume50
Issue number6 PART 2
DOIs
Publication statusPublished - 2011 Jun
Externally publishedYes

Fingerprint

DNA
Substrates
deoxyribonucleic acid
Metals
beads
metals
casts
Biomolecules
Polydimethylsiloxane
Polystyrenes
Thickness control
polystyrene
Thermal evaporation
Plasma etching
Sols
Field emission
Ozone
oxygen plasma
plasma etching
immobilization

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Production of double-layered metal nanocups for artificial nanospace of biomolecular reaction. / Kim, Hyonchol; Hayashi, Masahito; Terazono, Hideyuki; Takei, Hiroyuki; Yasuda, Kenji.

In: Japanese Journal of Applied Physics, Vol. 50, No. 6 PART 2, 06GJ03, 06.2011.

Research output: Contribution to journalArticle

Kim, Hyonchol ; Hayashi, Masahito ; Terazono, Hideyuki ; Takei, Hiroyuki ; Yasuda, Kenji. / Production of double-layered metal nanocups for artificial nanospace of biomolecular reaction. In: Japanese Journal of Applied Physics. 2011 ; Vol. 50, No. 6 PART 2.
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