Production of eicosapentaenoic acid by a bacterium isolated from mackerel intestines

Masamichi Akimoto, Tetsuya Ishii, Koji Yamagaki, Kazuhisa Ohtaguchi, Kozo Koide, Kazunaga Yazawa

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Optimization of culture conditions for the growth rate, 5,8,11,14,17-cis-Eicosapentaenoic acid (EPA) content and EPA productivity of a bacterium isolated from Pacific mackerel intestines was investigated by use of a culture medium containing 1.00 wt% peptone and 0.50 wt% yeast extract in an artificial sea water (ASW). Cultivation temperature affected the growth rate and cellular EPA content of the bacterium. The cellular EPA content at 8°C was as great as 16.8 mg/g of dry cells, which was more than two times greater than that at 25°C (7.3 mg/g of dry cells), although the growth rate showed a maximum at 25°C. Both the yield of bacterial cells and the cellular EPA content at 25°C reached maximum values when the pH of the culture medium was nearly 7.0 and when the concentration of ASW was 100% (v/v). Under optimum culture conditions [25°C pH 7.0 and 100% (v/v) ASW], the amount of EPA accumulated in the cellular lipids reached 45.6 mg/L of culture broth after 8 hr.

Original languageEnglish
Pages (from-to)911-915
Number of pages5
JournalJournal of the American Oil Chemists' Society
Volume67
Issue number12
DOIs
Publication statusPublished - 1990 Dec
Externally publishedYes

Fingerprint

Perciformes
Eicosapentaenoic Acid
mackerel
eicosapentaenoic acid
Intestines
Bacteria
intestines
Seawater
Acids
bacteria
NSC 153174
seawater
Culture Media
Growth
Water
culture media
Peptones
peptones
yeast extract
Yeast

Keywords

  • Alteromonas putrefaciens
  • eicosapentaenoic acid
  • mackerel
  • marine bacterium

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Food Science
  • Organic Chemistry
  • Chemical Engineering(all)

Cite this

Production of eicosapentaenoic acid by a bacterium isolated from mackerel intestines. / Akimoto, Masamichi; Ishii, Tetsuya; Yamagaki, Koji; Ohtaguchi, Kazuhisa; Koide, Kozo; Yazawa, Kazunaga.

In: Journal of the American Oil Chemists' Society, Vol. 67, No. 12, 12.1990, p. 911-915.

Research output: Contribution to journalArticle

Akimoto, M, Ishii, T, Yamagaki, K, Ohtaguchi, K, Koide, K & Yazawa, K 1990, 'Production of eicosapentaenoic acid by a bacterium isolated from mackerel intestines', Journal of the American Oil Chemists' Society, vol. 67, no. 12, pp. 911-915. https://doi.org/10.1007/BF02541846
Akimoto, Masamichi ; Ishii, Tetsuya ; Yamagaki, Koji ; Ohtaguchi, Kazuhisa ; Koide, Kozo ; Yazawa, Kazunaga. / Production of eicosapentaenoic acid by a bacterium isolated from mackerel intestines. In: Journal of the American Oil Chemists' Society. 1990 ; Vol. 67, No. 12. pp. 911-915.
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