Abstract
The eicosapentaenoic acid (EPA) synthesis gene cluster from an EPA-producing bacterium, Shewanella sp. SCRC-2738, was cloned into a broad-host range vector, pJRD215, and then introduced into a marine cyanobacterium, Synechococcus sp. NKBG15041c, by conjugation. The transconjugant cyanobacteria produced 3.7 ± 0.2% (2.24 ± 0.13 mg/L) EPA (n-3) and 2.5 ± 0.2% (1.49 ± 0.06 mg/L) eicosatetraenoic acid (n-3) of the total fatty acids when the cells were cultured at 23°C at a light intensity of 1,000-1,500 Lux. The EPA and eico-satetraenoic acid contents of the cells were increased to 4.6 ± 0.6% (3.86 ± 1.11 mg/L) and 4.7 ± 0.3% (3.86 ± 0.82 mg/L), and 7.5 ± 0.3% (1.76 ± 0.10 mg/L) and 5.1 ± 0.2% (1.19 ± 0.06 mg/L) when they were cultured at low temperature (18°C) and at lower light intensity (40 Lux), respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 1061-1064 |
| Number of pages | 4 |
| Journal | Lipids |
| Volume | 35 |
| Issue number | 10 |
| Publication status | Published - 2000 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Food Science
- Biochemistry
Cite this
Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp. / Yu, R.; Yamada, A.; Watanabe, K.; Yazawa, Kazunaga; Takeyama, Haruko; Matsunaga, Tadashi; Kurane, R.
In: Lipids, Vol. 35, No. 10, 2000, p. 1061-1064.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp.
AU - Yu, R.
AU - Yamada, A.
AU - Watanabe, K.
AU - Yazawa, Kazunaga
AU - Takeyama, Haruko
AU - Matsunaga, Tadashi
AU - Kurane, R.
PY - 2000
Y1 - 2000
N2 - The eicosapentaenoic acid (EPA) synthesis gene cluster from an EPA-producing bacterium, Shewanella sp. SCRC-2738, was cloned into a broad-host range vector, pJRD215, and then introduced into a marine cyanobacterium, Synechococcus sp. NKBG15041c, by conjugation. The transconjugant cyanobacteria produced 3.7 ± 0.2% (2.24 ± 0.13 mg/L) EPA (n-3) and 2.5 ± 0.2% (1.49 ± 0.06 mg/L) eicosatetraenoic acid (n-3) of the total fatty acids when the cells were cultured at 23°C at a light intensity of 1,000-1,500 Lux. The EPA and eico-satetraenoic acid contents of the cells were increased to 4.6 ± 0.6% (3.86 ± 1.11 mg/L) and 4.7 ± 0.3% (3.86 ± 0.82 mg/L), and 7.5 ± 0.3% (1.76 ± 0.10 mg/L) and 5.1 ± 0.2% (1.19 ± 0.06 mg/L) when they were cultured at low temperature (18°C) and at lower light intensity (40 Lux), respectively.
AB - The eicosapentaenoic acid (EPA) synthesis gene cluster from an EPA-producing bacterium, Shewanella sp. SCRC-2738, was cloned into a broad-host range vector, pJRD215, and then introduced into a marine cyanobacterium, Synechococcus sp. NKBG15041c, by conjugation. The transconjugant cyanobacteria produced 3.7 ± 0.2% (2.24 ± 0.13 mg/L) EPA (n-3) and 2.5 ± 0.2% (1.49 ± 0.06 mg/L) eicosatetraenoic acid (n-3) of the total fatty acids when the cells were cultured at 23°C at a light intensity of 1,000-1,500 Lux. The EPA and eico-satetraenoic acid contents of the cells were increased to 4.6 ± 0.6% (3.86 ± 1.11 mg/L) and 4.7 ± 0.3% (3.86 ± 0.82 mg/L), and 7.5 ± 0.3% (1.76 ± 0.10 mg/L) and 5.1 ± 0.2% (1.19 ± 0.06 mg/L) when they were cultured at low temperature (18°C) and at lower light intensity (40 Lux), respectively.
UR - http://www.scopus.com/inward/record.url?scp=0033744916&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033744916&partnerID=8YFLogxK
M3 - Article
C2 - 11104010
AN - SCOPUS:0033744916
VL - 35
SP - 1061
EP - 1064
JO - Lipids
JF - Lipids
SN - 0024-4201
IS - 10
ER -