Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp.

R. Yu, A. Yamada, K. Watanabe, Kazunaga Yazawa, Haruko Takeyama, Tadashi Matsunaga, R. Kurane

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The eicosapentaenoic acid (EPA) synthesis gene cluster from an EPA-producing bacterium, Shewanella sp. SCRC-2738, was cloned into a broad-host range vector, pJRD215, and then introduced into a marine cyanobacterium, Synechococcus sp. NKBG15041c, by conjugation. The transconjugant cyanobacteria produced 3.7 ± 0.2% (2.24 ± 0.13 mg/L) EPA (n-3) and 2.5 ± 0.2% (1.49 ± 0.06 mg/L) eicosatetraenoic acid (n-3) of the total fatty acids when the cells were cultured at 23°C at a light intensity of 1,000-1,500 Lux. The EPA and eico-satetraenoic acid contents of the cells were increased to 4.6 ± 0.6% (3.86 ± 1.11 mg/L) and 4.7 ± 0.3% (3.86 ± 0.82 mg/L), and 7.5 ± 0.3% (1.76 ± 0.10 mg/L) and 5.1 ± 0.2% (1.19 ± 0.06 mg/L) when they were cultured at low temperature (18°C) and at lower light intensity (40 Lux), respectively.

Original languageEnglish
Pages (from-to)1061-1064
Number of pages4
JournalLipids
Volume35
Issue number10
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Synechococcus
Eicosapentaenoic Acid
Cyanobacteria
eicosapentaenoic acid
light intensity
Shewanella
Arachidonic Acids
Light
Host Specificity
acids
Omega-3 Fatty Acids
Multigene Family
multigene family
host range
Cultured Cells
Bacteria
Fatty Acids
Genes
cells
fatty acids

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science
  • Biochemistry

Cite this

Yu, R., Yamada, A., Watanabe, K., Yazawa, K., Takeyama, H., Matsunaga, T., & Kurane, R. (2000). Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp. Lipids, 35(10), 1061-1064.

Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp. / Yu, R.; Yamada, A.; Watanabe, K.; Yazawa, Kazunaga; Takeyama, Haruko; Matsunaga, Tadashi; Kurane, R.

In: Lipids, Vol. 35, No. 10, 2000, p. 1061-1064.

Research output: Contribution to journalArticle

Yu, R, Yamada, A, Watanabe, K, Yazawa, K, Takeyama, H, Matsunaga, T & Kurane, R 2000, 'Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp.', Lipids, vol. 35, no. 10, pp. 1061-1064.
Yu R, Yamada A, Watanabe K, Yazawa K, Takeyama H, Matsunaga T et al. Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp. Lipids. 2000;35(10):1061-1064.
Yu, R. ; Yamada, A. ; Watanabe, K. ; Yazawa, Kazunaga ; Takeyama, Haruko ; Matsunaga, Tadashi ; Kurane, R. / Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp. In: Lipids. 2000 ; Vol. 35, No. 10. pp. 1061-1064.
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