Production of eicosapentaenoic acid from marine bacteria

Kazunaga Yazawa

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

A marine bacterium, judged as a new species close to Shewanells putrefaciens, was isolated from the intestinal contents of the Pacific mackerel. The isolated strain SCRC-2378 produced eicosapentaenoic acid (EPA) as the sole polyunsaturated fatty acid, which amounted to 24-40% of the total fatty acid in the cell, which corresponded to 2% of dry cell weight. Under the optimal growth conditions (pH 7.0, 20°C, and grown aerobically for 12-18 h), the yield of SCRC-2738 reached 15 g of dry cells/L or 2 × 1010 viable cells/mL. EPA existed as phospholipid and was found in the sn-2 position of phosphatidylethanolamine and phosphatidylglycerol. The 38 kbp (1,000 base pairs) genome DNA fragment was cloned from SCRC-2738 and expressed in Escherichia coli, which resulted in the production of EPA. The nucleotide sequence of the 38 kbp DNA fragment was determined. The DNA fragment contains eight open reading frames, and three of them possess homology with enzymes involved in fatty acid synthesis. Thus, it may be possible that these EPA biosynthesis genes are applied for EPA production in yeasts or higher plants, and offer a new method for EPA synthesis as new foods containing EPA.

Original languageEnglish
JournalLipids
Volume31
Issue number3 SUPPL.
Publication statusPublished - 1996 Mar
Externally publishedYes

Fingerprint

Eicosapentaenoic Acid
eicosapentaenoic acid
Bacteria
bacteria
DNA
Fatty Acids
Genes
cells
fatty acids
Perciformes
Phosphatidylglycerols
Gastrointestinal Contents
synthesis
phosphatidylglycerols
Biosynthesis
mackerel
phosphatidylethanolamines
Unsaturated Fatty Acids
Base Pairing
Yeast

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Medicine (miscellaneous)

Cite this

Production of eicosapentaenoic acid from marine bacteria. / Yazawa, Kazunaga.

In: Lipids, Vol. 31, No. 3 SUPPL., 03.1996.

Research output: Contribution to journalArticle

Yazawa, K 1996, 'Production of eicosapentaenoic acid from marine bacteria', Lipids, vol. 31, no. 3 SUPPL..
Yazawa, Kazunaga. / Production of eicosapentaenoic acid from marine bacteria. In: Lipids. 1996 ; Vol. 31, No. 3 SUPPL.
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