Production of Immunoabsorbent Nanoparticles by Displaying Single-Domain Protein A on Potato Virus X

Kerstin Uhde-Holzem, Michael McBurney, Brylee David B. Tiu, Rigoberto C. Advincula, Rainer Fischer, Ulrich Commandeur, Nicole F. Steinmetz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The combination of antibodies with nanoparticles provides wide-ranging applications in biosensing. While several covalent presentation strategies have been established, there is need for alternative, non-covalent methods to provide a routine for scalable nanomanufacturing. We report the multivalent presentation of the B domain of Staphylococcus aureus protein A (SpAB) on potato virus X (PVX) nanoparticles. Three different synthetic strategies were used to obtain chimeric PVXSpAB filaments. The protein A fragments displayed on the surface of all three PVX chimeras remained fully functional as an immunoabsorbent for antibody capture enabling biosensing. The new biomaterials presented could find applications as diagnostic tools for biomedical or environmental monitoring.

Original languageEnglish
Pages (from-to)231-241
Number of pages11
JournalMacromolecular Bioscience
Volume16
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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  • Cite this

    Uhde-Holzem, K., McBurney, M., Tiu, B. D. B., Advincula, R. C., Fischer, R., Commandeur, U., & Steinmetz, N. F. (2016). Production of Immunoabsorbent Nanoparticles by Displaying Single-Domain Protein A on Potato Virus X. Macromolecular Bioscience, 16(2), 231-241. https://doi.org/10.1002/mabi.201500280