Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Tomoharu Osada, Atsushi Toyoda, Stefan Moisyadi, Hidenori Akutsu, Masahira Hattori, Yoshiyuio Sakaki, Ryuzo Yanagimachi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

Original languageEnglish
Pages (from-to)329-335
Number of pages7
JournalMolecular Reproduction and Development
Volume72
Issue number3
DOIs
Publication statusPublished - 2005 Nov
Externally publishedYes

Keywords

  • Bacterial artificial chromosome (BAC)
  • Down syndrome
  • Inbred mice
  • Intracytoplasmic sperm injection (ICSI)
  • Transgenesis

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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