Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Tomoharu Osada, Atsushi Toyoda, Stefan Moisyadi, Hidenori Akutsu, Masahira Hattori, Yoshiyuio Sakaki, Ryuzo Yanagimachi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

Original languageEnglish
Pages (from-to)329-335
Number of pages7
JournalMolecular Reproduction and Development
Volume72
Issue number3
DOIs
Publication statusPublished - 2005 Nov
Externally publishedYes

Fingerprint

Intracytoplasmic Sperm Injections
Transgenic Mice
Bacterial Artificial Chromosomes
Chromosomes, Human, Pair 21
Gene Transfer Techniques
DNA
Human Chromosomes
Base Pairing
Clone Cells
Genome
Technology
Genes
Down Syndrome Critical Region

Keywords

  • Bacterial artificial chromosome (BAC)
  • Down syndrome
  • Inbred mice
  • Intracytoplasmic sperm injection (ICSI)
  • Transgenesis

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection. / Osada, Tomoharu; Toyoda, Atsushi; Moisyadi, Stefan; Akutsu, Hidenori; Hattori, Masahira; Sakaki, Yoshiyuio; Yanagimachi, Ryuzo.

In: Molecular Reproduction and Development, Vol. 72, No. 3, 11.2005, p. 329-335.

Research output: Contribution to journalArticle

Osada, Tomoharu ; Toyoda, Atsushi ; Moisyadi, Stefan ; Akutsu, Hidenori ; Hattori, Masahira ; Sakaki, Yoshiyuio ; Yanagimachi, Ryuzo. / Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection. In: Molecular Reproduction and Development. 2005 ; Vol. 72, No. 3. pp. 329-335.
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