Abstract
We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.
Original language | English |
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Pages (from-to) | 329-335 |
Number of pages | 7 |
Journal | Molecular Reproduction and Development |
Volume | 72 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2005 Nov |
Externally published | Yes |
Keywords
- Bacterial artificial chromosome (BAC)
- Down syndrome
- Inbred mice
- Intracytoplasmic sperm injection (ICSI)
- Transgenesis
ASJC Scopus subject areas
- Genetics
- Developmental Biology
- Cell Biology