Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Tomoharu Osada; Atsushi Toyoda; Stefan Moisyadi; Hidenori Akutsu; Masahira Hattori; Yoshiyuio Sakaki; Ryuzo Yanagimachi

doi:10.1002/mrd.20319

Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Tomoharu Osada^*, Atsushi Toyoda, Stefan Moisyadi, Hidenori Akutsu, Masahira Hattori, Yoshiyuio Sakaki, Ryuzo Yanagimachi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

Original language	English
Pages (from-to)	329-335
Number of pages	7
Journal	Molecular Reproduction and Development
Volume	72
Issue number	3
DOIs	https://doi.org/10.1002/mrd.20319
Publication status	Published - 2005 Nov
Externally published	Yes

Keywords

Bacterial artificial chromosome (BAC)
Down syndrome
Inbred mice
Intracytoplasmic sperm injection (ICSI)
Transgenesis

ASJC Scopus subject areas

Genetics
Developmental Biology
Cell Biology

Access to Document

10.1002/mrd.20319

Cite this

@article{395e773169b747d9bec794f5ef8f6264,

title = "Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection",

abstract = "We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.",

keywords = "Bacterial artificial chromosome (BAC), Down syndrome, Inbred mice, Intracytoplasmic sperm injection (ICSI), Transgenesis",

author = "Tomoharu Osada and Atsushi Toyoda and Stefan Moisyadi and Hidenori Akutsu and Masahira Hattori and Yoshiyuio Sakaki and Ryuzo Yanagimachi",

year = "2005",

month = nov,

doi = "10.1002/mrd.20319",

language = "English",

volume = "72",

pages = "329--335",

journal = "Gamete Research",

issn = "1040-452X",

publisher = "Wiley-Liss Inc.",

number = "3",

}

TY - JOUR

T1 - Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

AU - Osada, Tomoharu

AU - Toyoda, Atsushi

AU - Moisyadi, Stefan

AU - Akutsu, Hidenori

AU - Hattori, Masahira

AU - Sakaki, Yoshiyuio

AU - Yanagimachi, Ryuzo

PY - 2005/11

Y1 - 2005/11

N2 - We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

AB - We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

KW - Bacterial artificial chromosome (BAC)

KW - Down syndrome

KW - Inbred mice

KW - Intracytoplasmic sperm injection (ICSI)

KW - Transgenesis

UR - http://www.scopus.com/inward/record.url?scp=25444515938&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25444515938&partnerID=8YFLogxK

U2 - 10.1002/mrd.20319

DO - 10.1002/mrd.20319

M3 - Article

C2 - 16047391

AN - SCOPUS:25444515938

VL - 72

SP - 329

EP - 335

JO - Gamete Research

JF - Gamete Research

SN - 1040-452X

IS - 3

ER -

Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this