Abstract
We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.
| Original language | English |
|---|---|
| Pages (from-to) | 329-335 |
| Number of pages | 7 |
| Journal | Molecular Reproduction and Development |
| Volume | 72 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2005 Nov |
| Externally published | Yes |
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Keywords
- Bacterial artificial chromosome (BAC)
- Down syndrome
- Inbred mice
- Intracytoplasmic sperm injection (ICSI)
- Transgenesis
ASJC Scopus subject areas
- Genetics
- Developmental Biology
- Cell Biology
Cite this
Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection. / Osada, Tomoharu; Toyoda, Atsushi; Moisyadi, Stefan; Akutsu, Hidenori; Hattori, Masahira; Sakaki, Yoshiyuio; Yanagimachi, Ryuzo.
In: Molecular Reproduction and Development, Vol. 72, No. 3, 11.2005, p. 329-335.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection
AU - Osada, Tomoharu
AU - Toyoda, Atsushi
AU - Moisyadi, Stefan
AU - Akutsu, Hidenori
AU - Hattori, Masahira
AU - Sakaki, Yoshiyuio
AU - Yanagimachi, Ryuzo
PY - 2005/11
Y1 - 2005/11
N2 - We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.
AB - We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.
KW - Bacterial artificial chromosome (BAC)
KW - Down syndrome
KW - Inbred mice
KW - Intracytoplasmic sperm injection (ICSI)
KW - Transgenesis
UR - http://www.scopus.com/inward/record.url?scp=25444515938&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=25444515938&partnerID=8YFLogxK
U2 - 10.1002/mrd.20319
DO - 10.1002/mrd.20319
M3 - Article
C2 - 16047391
AN - SCOPUS:25444515938
VL - 72
SP - 329
EP - 335
JO - Molecular Reproduction and Development
JF - Molecular Reproduction and Development
SN - 1040-452X
IS - 3
ER -