Abstract
We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.
| Original language | English |
|---|---|
| Pages (from-to) | 329-335 |
| Number of pages | 7 |
| Journal | Molecular Reproduction and Development |
| Volume | 72 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2005 Nov |
| Externally published | Yes |
Keywords
- Bacterial artificial chromosome (BAC)
- Down syndrome
- Inbred mice
- Intracytoplasmic sperm injection (ICSI)
- Transgenesis
ASJC Scopus subject areas
- Genetics
- Developmental Biology
- Cell Biology
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Cite this
Osada, T., Toyoda, A., Moisyadi, S., Akutsu, H., Hattori, M., Sakaki, Y., & Yanagimachi, R. (2005). Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection. Molecular Reproduction and Development, 72(3), 329-335. https://doi.org/10.1002/mrd.20319