Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Tomoharu Osada; Atsushi Toyoda; Stefan Moisyadi; Hidenori Akutsu; Masahira Hattori; Yoshiyuio Sakaki; Ryuzo Yanagimachi

doi:10.1002/mrd.20319

Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Tomoharu Osada^*, Atsushi Toyoda, Stefan Moisyadi, Hidenori Akutsu, Masahira Hattori, Yoshiyuio Sakaki, Ryuzo Yanagimachi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

Original language	English
Pages (from-to)	329-335
Number of pages	7
Journal	Molecular Reproduction and Development
Volume	72
Issue number	3
DOIs	https://doi.org/10.1002/mrd.20319
Publication status	Published - 2005 Nov
Externally published	Yes

Keywords

Bacterial artificial chromosome (BAC)
Down syndrome
Inbred mice
Intracytoplasmic sperm injection (ICSI)
Transgenesis

ASJC Scopus subject areas

Genetics
Developmental Biology
Cell Biology

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10.1002/mrd.20319

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Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection. / Osada, Tomoharu; Toyoda, Atsushi; Moisyadi, Stefan; Akutsu, Hidenori; Hattori, Masahira; Sakaki, Yoshiyuio; Yanagimachi, Ryuzo.

In: Molecular Reproduction and Development, Vol. 72, No. 3, 11.2005, p. 329-335.

Research output: Contribution to journal › Article › peer-review

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AU - Hattori, Masahira

AU - Sakaki, Yoshiyuio

AU - Yanagimachi, Ryuzo

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AB - We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

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Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Abstract

Keywords

ASJC Scopus subject areas

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