Abstract
Resting cells of a double mutant noted as KSM-MT66, derived from Rhodococcus sp. strain KSM-B-3 by UV irradiation, were found to cis-desaturate isopropyl hexadecanoate, yielding isopropyl cis-6-hexadecenoate. Addition of sodium glutamate (1.0%), MgSO 4 (2 mM), and thiamine (2 mM) increased the productivity of the unsaturated product in phosphate buffer. Optimal temperature and pH for the reaction were around 26°C and 7, respectively. Under the optimized conditions, more than 50 g/l of isopropyl cis-6-hexadecenoate was produced after a 3-day incubation by resting cells of the mutant. Thus, cis-6-hexadecenoic acid, the main component of human sebaceous lipids, can be manufactured economically by the rhodococcal bioconversion.
| Original language | English |
|---|---|
| Pages (from-to) | 399-404 |
| Number of pages | 6 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 64 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2000 Jan 1 |
| Externally published | Yes |
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Keywords
- Cis-6-hexadecenoic acid
- Desaturase
- Regiospecificity
- Rhodococcus
- Sebaceous lipid
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
Production of Isopropyl cis-6-Hexadecenoate by Regiospecific Desaturation of Isopropyl Palmitate by a Double Mutant of a Rhodococcus Strain. / Koike, Kenzo; Takaiwa, Mikio; Ara, Katsutoshi; Inoue, Shigeo; Kimura, Yoshiharu; Ito, Susumu.
In: Bioscience, Biotechnology and Biochemistry, Vol. 64, No. 2, 01.01.2000, p. 399-404.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of Isopropyl cis-6-Hexadecenoate by Regiospecific Desaturation of Isopropyl Palmitate by a Double Mutant of a Rhodococcus Strain
AU - Koike, Kenzo
AU - Takaiwa, Mikio
AU - Ara, Katsutoshi
AU - Inoue, Shigeo
AU - Kimura, Yoshiharu
AU - Ito, Susumu
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Resting cells of a double mutant noted as KSM-MT66, derived from Rhodococcus sp. strain KSM-B-3 by UV irradiation, were found to cis-desaturate isopropyl hexadecanoate, yielding isopropyl cis-6-hexadecenoate. Addition of sodium glutamate (1.0%), MgSO 4 (2 mM), and thiamine (2 mM) increased the productivity of the unsaturated product in phosphate buffer. Optimal temperature and pH for the reaction were around 26°C and 7, respectively. Under the optimized conditions, more than 50 g/l of isopropyl cis-6-hexadecenoate was produced after a 3-day incubation by resting cells of the mutant. Thus, cis-6-hexadecenoic acid, the main component of human sebaceous lipids, can be manufactured economically by the rhodococcal bioconversion.
AB - Resting cells of a double mutant noted as KSM-MT66, derived from Rhodococcus sp. strain KSM-B-3 by UV irradiation, were found to cis-desaturate isopropyl hexadecanoate, yielding isopropyl cis-6-hexadecenoate. Addition of sodium glutamate (1.0%), MgSO 4 (2 mM), and thiamine (2 mM) increased the productivity of the unsaturated product in phosphate buffer. Optimal temperature and pH for the reaction were around 26°C and 7, respectively. Under the optimized conditions, more than 50 g/l of isopropyl cis-6-hexadecenoate was produced after a 3-day incubation by resting cells of the mutant. Thus, cis-6-hexadecenoic acid, the main component of human sebaceous lipids, can be manufactured economically by the rhodococcal bioconversion.
KW - Cis-6-hexadecenoic acid
KW - Desaturase
KW - Regiospecificity
KW - Rhodococcus
KW - Sebaceous lipid
UR - http://www.scopus.com/inward/record.url?scp=0034133121&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034133121&partnerID=8YFLogxK
U2 - 10.1271/bbb.64.399
DO - 10.1271/bbb.64.399
M3 - Article
C2 - 10737199
AN - SCOPUS:0034133121
VL - 64
SP - 399
EP - 404
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 2
ER -