Abstract
Resting cells of a double mutant noted as KSM-MT66, derived from Rhodococcus sp. strain KSM-B-3 by UV irradiation, were found to cis-desaturate isopropyl hexadecanoate, yielding isopropyl cis-6-hexadecenoate. Addition of sodium glutamate (1.0%), MgSO4 (2 mM), and thiamine (2 mM) increased the productivity of the unsaturated product in phosphate buffer. Optimal temperature and pH for the reaction were around 26°C and 7, respectively. Under the optimized conditions, more than 50 g/l of isopropyl cis-6-hexadecenoate was produced after a 3-day incubation by resting cells of the mutant. Thus, cis-6-hexadecenoic acid, the main component of human sebaceous lipids, can be manufactured economically by the rhodococcal bioconversion.
| Original language | English |
|---|---|
| Pages (from-to) | 399-404 |
| Number of pages | 6 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 64 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2000 Jan 1 |
| Externally published | Yes |
Keywords
- Cis-6-hexadecenoic acid
- Desaturase
- Regiospecificity
- Rhodococcus
- Sebaceous lipid
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
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Cite this
Koike, K., Takaiwa, M., Ara, K., Inoue, S., Kimura, Y., & Ito, S. (2000). Production of Isopropyl cis-6-Hexadecenoate by Regiospecific Desaturation of Isopropyl Palmitate by a Double Mutant of a Rhodococcus Strain. Bioscience, Biotechnology and Biochemistry, 64(2), 399-404. https://doi.org/10.1271/bbb.64.399