Production of Isopropyl cis-6-Hexadecenoate by Regiospecific Desaturation of Isopropyl Palmitate by a Double Mutant of a Rhodococcus Strain

Kenzo Koike, Mikio Takaiwa, Katsutoshi Ara, Shigeo Inoue, Yoshiharu Kimura, Susumu Ito

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Resting cells of a double mutant noted as KSM-MT66, derived from Rhodococcus sp. strain KSM-B-3 by UV irradiation, were found to cis-desaturate isopropyl hexadecanoate, yielding isopropyl cis-6-hexadecenoate. Addition of sodium glutamate (1.0%), MgSO4 (2 mM), and thiamine (2 mM) increased the productivity of the unsaturated product in phosphate buffer. Optimal temperature and pH for the reaction were around 26°C and 7, respectively. Under the optimized conditions, more than 50 g/l of isopropyl cis-6-hexadecenoate was produced after a 3-day incubation by resting cells of the mutant. Thus, cis-6-hexadecenoic acid, the main component of human sebaceous lipids, can be manufactured economically by the rhodococcal bioconversion.

Original languageEnglish
Pages (from-to)399-404
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume64
Issue number2
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Keywords

  • Cis-6-hexadecenoic acid
  • Desaturase
  • Regiospecificity
  • Rhodococcus
  • Sebaceous lipid

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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