Production of p-aminosalicylic acid through enzymatic kolbeschmitt reaction catalyzed by reversible salicylic acid decarboxylase

Kohtaro Kirimura; Satomi Yanaso; Sachiyo Kosaka; Keiko Koyama; Takasumi Hattori; Yoshitaka Ishii

doi:10.1246/cl.2011.206

Production of p-aminosalicylic acid through enzymatic kolbeschmitt reaction catalyzed by reversible salicylic acid decarboxylase

Kohtaro Kirimura^*, Satomi Yanaso, Sachiyo Kosaka, Keiko Koyama, Takasumi Hattori, Yoshitaka Ishii

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

A reversible salicylic acid decarboxylase (Sdc), found in the yeast Trichosporon moniliiforme WU-0401, is applicable for the production of p-aminosalicylic acid (PAS) from m-aminophenol (m-AP). For the high-yield production of PAS, used as an antituberculous agent, we developed F195Y, a genetically engineered Sdc mutant. We succeeded in selectively producing PAS from m-AP through an enzymatic KolbeSchmitt reaction in aqueous solution by using recombinant Escherichia coli cells expressing the gene encoding F195Y. We found that 70mM PAS was produced at 30 °C in 15h with a conversion yield of 70% (mol/mol).

Original language	English
Pages (from-to)	206-208
Number of pages	3
Journal	Chemistry Letters
Volume	40
Issue number	2
DOIs	https://doi.org/10.1246/cl.2011.206
Publication status	Published - 2011

ASJC Scopus subject areas

Chemistry(all)

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abstract = "A reversible salicylic acid decarboxylase (Sdc), found in the yeast Trichosporon moniliiforme WU-0401, is applicable for the production of p-aminosalicylic acid (PAS) from m-aminophenol (m-AP). For the high-yield production of PAS, used as an antituberculous agent, we developed F195Y, a genetically engineered Sdc mutant. We succeeded in selectively producing PAS from m-AP through an enzymatic KolbeSchmitt reaction in aqueous solution by using recombinant Escherichia coli cells expressing the gene encoding F195Y. We found that 70mM PAS was produced at 30 °C in 15h with a conversion yield of 70% (mol/mol).",

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T1 - Production of p-aminosalicylic acid through enzymatic kolbeschmitt reaction catalyzed by reversible salicylic acid decarboxylase

AU - Kirimura, Kohtaro

AU - Yanaso, Satomi

AU - Kosaka, Sachiyo

AU - Koyama, Keiko

AU - Hattori, Takasumi

AU - Ishii, Yoshitaka

PY - 2011

Y1 - 2011

N2 - A reversible salicylic acid decarboxylase (Sdc), found in the yeast Trichosporon moniliiforme WU-0401, is applicable for the production of p-aminosalicylic acid (PAS) from m-aminophenol (m-AP). For the high-yield production of PAS, used as an antituberculous agent, we developed F195Y, a genetically engineered Sdc mutant. We succeeded in selectively producing PAS from m-AP through an enzymatic KolbeSchmitt reaction in aqueous solution by using recombinant Escherichia coli cells expressing the gene encoding F195Y. We found that 70mM PAS was produced at 30 °C in 15h with a conversion yield of 70% (mol/mol).

AB - A reversible salicylic acid decarboxylase (Sdc), found in the yeast Trichosporon moniliiforme WU-0401, is applicable for the production of p-aminosalicylic acid (PAS) from m-aminophenol (m-AP). For the high-yield production of PAS, used as an antituberculous agent, we developed F195Y, a genetically engineered Sdc mutant. We succeeded in selectively producing PAS from m-AP through an enzymatic KolbeSchmitt reaction in aqueous solution by using recombinant Escherichia coli cells expressing the gene encoding F195Y. We found that 70mM PAS was produced at 30 °C in 15h with a conversion yield of 70% (mol/mol).

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Production of p-aminosalicylic acid through enzymatic kolbeschmitt reaction catalyzed by reversible salicylic acid decarboxylase

Abstract

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