Production of p-aminosalicylic acid through enzymatic kolbeschmitt reaction catalyzed by reversible salicylic acid decarboxylase

Kotaro Kirimura, Satomi Yanaso, Sachiyo Kosaka, Keiko Koyama, Takasumi Hattori, Yoshitaka Ishii

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    A reversible salicylic acid decarboxylase (Sdc), found in the yeast Trichosporon moniliiforme WU-0401, is applicable for the production of p-aminosalicylic acid (PAS) from m-aminophenol (m-AP). For the high-yield production of PAS, used as an antituberculous agent, we developed F195Y, a genetically engineered Sdc mutant. We succeeded in selectively producing PAS from m-AP through an enzymatic KolbeSchmitt reaction in aqueous solution by using recombinant Escherichia coli cells expressing the gene encoding F195Y. We found that 70mM PAS was produced at 30 °C in 15h with a conversion yield of 70% (mol/mol).

    Original languageEnglish
    Pages (from-to)206-208
    Number of pages3
    JournalChemistry Letters
    Volume40
    Issue number2
    DOIs
    Publication statusPublished - 2011

    Fingerprint

    Aminosalicylic Acid
    Carboxy-Lyases
    Salicylic Acid
    Gene encoding
    Yeast
    Escherichia coli
    Cells

    ASJC Scopus subject areas

    • Chemistry(all)

    Cite this

    Production of p-aminosalicylic acid through enzymatic kolbeschmitt reaction catalyzed by reversible salicylic acid decarboxylase. / Kirimura, Kotaro; Yanaso, Satomi; Kosaka, Sachiyo; Koyama, Keiko; Hattori, Takasumi; Ishii, Yoshitaka.

    In: Chemistry Letters, Vol. 40, No. 2, 2011, p. 206-208.

    Research output: Contribution to journalArticle

    Kirimura, Kotaro ; Yanaso, Satomi ; Kosaka, Sachiyo ; Koyama, Keiko ; Hattori, Takasumi ; Ishii, Yoshitaka. / Production of p-aminosalicylic acid through enzymatic kolbeschmitt reaction catalyzed by reversible salicylic acid decarboxylase. In: Chemistry Letters. 2011 ; Vol. 40, No. 2. pp. 206-208.
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    AU - Koyama, Keiko

    AU - Hattori, Takasumi

    AU - Ishii, Yoshitaka

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