TY - JOUR
T1 - Productivity and bioactivity of enokipodins A–D of Flammulina rossica and Flammulina velutipes
AU - Tabuchi, Akiko
AU - Fukushima-Sakuno, Emi
AU - Osaki-Oka, Kumiko
AU - Futamura, Yushi
AU - Motoyama, Takayuki
AU - Osada, Hiroyuki
AU - Ishikawa, Noemia Kazue
AU - Nagasawa, Eiji
AU - Tokimoto, Keisuke
N1 - Funding Information:
We thank H. Hayase and H. Aono (Chem Biol, RIKEN CSRS) for technical support. We also thank Y. Uehara (Iwate Medical University) for providing src ts-NRK cells.
Publisher Copyright:
© 2020, © 2020 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2020/5/3
Y1 - 2020/5/3
N2 - Enokipodins are antimicrobial sesquiterpenes produced by Flammulina velutipes in a mycelial culture medium. To date, enokipodin production has not been reported in other members of the genus Flammulina. Hence, in this study, the production of enokipodins A, B, C, and D by F. velutipes and F. rossica was investigated. Some strains of F. rossica were confirmed to produce at least one of the four enokipodins in the culture medium. However, some strains of F. velutipes did not produce any of the enokipodins. In an antibacterial assay using liquid medium, enokipodin B showed the strongest growth inhibitory activity against Bacillus subtilis among the four types of enokipodins. Enokipodin B inhibited the spore germination of some plant pathogenic fungi. Enokipodins B and D exerted moderate anti-proliferative activity against some cancer cell lines, and enokipodins A and C inhibited the proliferation of the malarial parasite, Plasmodium falciparum.
AB - Enokipodins are antimicrobial sesquiterpenes produced by Flammulina velutipes in a mycelial culture medium. To date, enokipodin production has not been reported in other members of the genus Flammulina. Hence, in this study, the production of enokipodins A, B, C, and D by F. velutipes and F. rossica was investigated. Some strains of F. rossica were confirmed to produce at least one of the four enokipodins in the culture medium. However, some strains of F. velutipes did not produce any of the enokipodins. In an antibacterial assay using liquid medium, enokipodin B showed the strongest growth inhibitory activity against Bacillus subtilis among the four types of enokipodins. Enokipodin B inhibited the spore germination of some plant pathogenic fungi. Enokipodins B and D exerted moderate anti-proliferative activity against some cancer cell lines, and enokipodins A and C inhibited the proliferation of the malarial parasite, Plasmodium falciparum.
KW - Enokipodins
KW - Flammulina rossica
KW - Flammulina velutipes
KW - bioactivity
KW - productivity
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U2 - 10.1080/09168451.2020.1714421
DO - 10.1080/09168451.2020.1714421
M3 - Article
C2 - 31942814
AN - SCOPUS:85078635173
SN - 0916-8451
VL - 84
SP - 876
EP - 886
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 5
ER -