Productivity and bioactivity of enokipodins A–D of Flammulina rossica and Flammulina velutipes

Akiko Tabuchi, Emi Fukushima-Sakuno*, Kumiko Osaki-Oka, Yushi Futamura, Takayuki Motoyama, Hiroyuki Osada, Noemia Kazue Ishikawa, Eiji Nagasawa, Keisuke Tokimoto

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Enokipodins are antimicrobial sesquiterpenes produced by Flammulina velutipes in a mycelial culture medium. To date, enokipodin production has not been reported in other members of the genus Flammulina. Hence, in this study, the production of enokipodins A, B, C, and D by F. velutipes and F. rossica was investigated. Some strains of F. rossica were confirmed to produce at least one of the four enokipodins in the culture medium. However, some strains of F. velutipes did not produce any of the enokipodins. In an antibacterial assay using liquid medium, enokipodin B showed the strongest growth inhibitory activity against Bacillus subtilis among the four types of enokipodins. Enokipodin B inhibited the spore germination of some plant pathogenic fungi. Enokipodins B and D exerted moderate anti-proliferative activity against some cancer cell lines, and enokipodins A and C inhibited the proliferation of the malarial parasite, Plasmodium falciparum.

Original languageEnglish
Pages (from-to)876-886
Number of pages11
JournalBioscience, Biotechnology and Biochemistry
Issue number5
Publication statusPublished - 2020 May 3
Externally publishedYes


  • Enokipodins
  • Flammulina rossica
  • Flammulina velutipes
  • bioactivity
  • productivity

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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