Profiling of Circulating MicroRNAs after a Bout of Acute Resistance Exercise in Humans

Shuji Sawada, Michihiro Kon, Shogo Wada, Takashi Ushida, Katsuhiko Suzuki, Takayuki Akimoto

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Recent studies have revealed a new aspect of physiological regulation in which microRNAs (miRNAs) play fundamental roles in diverse biological and pathological processes. Furthermore, it was recently discovered that miRNAs are stably secreted into blood and that circulating miRNAs may play important roles in cell-cell communication. Here, we examined whether the circulating miRNA profile is affected by acute resistance exercise. Twelve males performed a resistance exercise session (bench press and leg press), consisting of five sets of 10 repetitions at 70% of maximum strength, with a 1 min rest between sets. Blood samples were taken before exercise, and at 0 and 60 min, 1 day, and 3 days after exercise. The circulating miRNA profile was determined by microarray analysis. Quantitative real-time PCR confirmed that the miR-149* level increased three days after resistance exercise. In contrast, the miR-146a and miR-221 levels decreased three days after resistance exercise. Our findings suggest that circulating miRNA levels change in response to acute resistance exercise, and miRNAs may play important roles in resistance-exercise-induced adaptation.

Original languageEnglish
Article numbere70823
JournalPLoS One
Volume8
Issue number7
DOIs
Publication statusPublished - 2013 Jul 29

Fingerprint

strength training
MicroRNAs
microRNA
Exercise
exercise
Blood
Biological Phenomena
physiological regulation
cell communication
blood
Pathologic Processes
Microarray Analysis
Microarrays
Cell Communication
Real-Time Polymerase Chain Reaction
Leg
legs
quantitative polymerase chain reaction
Communication

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Profiling of Circulating MicroRNAs after a Bout of Acute Resistance Exercise in Humans. / Sawada, Shuji; Kon, Michihiro; Wada, Shogo; Ushida, Takashi; Suzuki, Katsuhiko; Akimoto, Takayuki.

In: PLoS One, Vol. 8, No. 7, e70823, 29.07.2013.

Research output: Contribution to journalArticle

Sawada, Shuji ; Kon, Michihiro ; Wada, Shogo ; Ushida, Takashi ; Suzuki, Katsuhiko ; Akimoto, Takayuki. / Profiling of Circulating MicroRNAs after a Bout of Acute Resistance Exercise in Humans. In: PLoS One. 2013 ; Vol. 8, No. 7.
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