Abstract
Effect of prolactin on the testicular luteinizing hormone binding was studied in a serum-free culture system. By the collagenase digestion of decapsulated testes taken out from 25-day-old rats, Leydig cells were isolated and cultured for 7 days in DME/F12 (1:1) medium supplemented with insulin, transferrin, epidermal growth factor, and gentamicin. The cultured cells exhibited the 3β-hydroxysteroid dehydrogenase activity. Hill plots constructed from the data of competition experiment showed that the dissociation constant (Kd) was 0.33 × 10-10M. The Kd value was approximately the same as the known value for the rat testicular homogenates. When the Leydig cells were cultured with ovine prolactin for the last 3 days of 7-day culture period, the binding of luteinizing hormone increased to 1.7-fold ofthat in the control group. From these results it is concluded that prolactin acts to up-regulate the binding of luteinizing hormone to rat testicular Leydig cells in serum-free culture
| Original language | English |
|---|---|
| Pages (from-to) | 511-517 |
| Number of pages | 7 |
| Journal | Journal of Biosciences |
| Volume | 21 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1996 Jun |
| Externally published | Yes |
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Keywords
- Leydig cell
- luteinizing hormone binding
- Prolactin
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
Cite this
Prolactin augments luteinizing hormone binding to rat Leydig cells in serum-free culture. / Takase, Minoru; Tsutsui, Kazuyoshi; Kawashima, Seiichiro.
In: Journal of Biosciences, Vol. 21, No. 4, 06.1996, p. 511-517.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prolactin augments luteinizing hormone binding to rat Leydig cells in serum-free culture
AU - Takase, Minoru
AU - Tsutsui, Kazuyoshi
AU - Kawashima, Seiichiro
PY - 1996/6
Y1 - 1996/6
N2 - Effect of prolactin on the testicular luteinizing hormone binding was studied in a serum-free culture system. By the collagenase digestion of decapsulated testes taken out from 25-day-old rats, Leydig cells were isolated and cultured for 7 days in DME/F12 (1:1) medium supplemented with insulin, transferrin, epidermal growth factor, and gentamicin. The cultured cells exhibited the 3β-hydroxysteroid dehydrogenase activity. Hill plots constructed from the data of competition experiment showed that the dissociation constant (Kd) was 0.33 × 10-10M. The Kd value was approximately the same as the known value for the rat testicular homogenates. When the Leydig cells were cultured with ovine prolactin for the last 3 days of 7-day culture period, the binding of luteinizing hormone increased to 1.7-fold ofthat in the control group. From these results it is concluded that prolactin acts to up-regulate the binding of luteinizing hormone to rat testicular Leydig cells in serum-free culture
AB - Effect of prolactin on the testicular luteinizing hormone binding was studied in a serum-free culture system. By the collagenase digestion of decapsulated testes taken out from 25-day-old rats, Leydig cells were isolated and cultured for 7 days in DME/F12 (1:1) medium supplemented with insulin, transferrin, epidermal growth factor, and gentamicin. The cultured cells exhibited the 3β-hydroxysteroid dehydrogenase activity. Hill plots constructed from the data of competition experiment showed that the dissociation constant (Kd) was 0.33 × 10-10M. The Kd value was approximately the same as the known value for the rat testicular homogenates. When the Leydig cells were cultured with ovine prolactin for the last 3 days of 7-day culture period, the binding of luteinizing hormone increased to 1.7-fold ofthat in the control group. From these results it is concluded that prolactin acts to up-regulate the binding of luteinizing hormone to rat testicular Leydig cells in serum-free culture
KW - Leydig cell
KW - luteinizing hormone binding
KW - Prolactin
UR - http://www.scopus.com/inward/record.url?scp=4243167208&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4243167208&partnerID=8YFLogxK
U2 - 10.1007/BF02703215
DO - 10.1007/BF02703215
M3 - Article
AN - SCOPUS:4243167208
VL - 21
SP - 511
EP - 517
JO - Journal of Biosciences
JF - Journal of Biosciences
SN - 0250-5991
IS - 4
ER -