Proliferation related acidic leucine-rich protein PAL31 functions as a caspase-3 inhibitor

Weiyong Sun, Hiromichi Kimura, Naka Hattori, Satoshi Tanaka, Shigemi Matsuyama, Kunio Shiota

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Proliferation related acidic leucine-rich protein PAL31 (PAL31) is expressed in proliferating cells and consists of 272 amino acids with a tandem structure of leucine-rich repeats in the N-terminus and a highly acidic region with a putative nuclear localization signal in the C-terminus. We previously reported that PAL31 is required for cell cycle progression. In the present study, we found that the antisense oligonucleotide of PAL31 induced apoptosis to the transfected Nb2 cells. Stable transfectants, in which PAL31 was regulated by an inducible promoter, were generated to gain further insight into the signaling role of PAL31 in the regulation of apoptosis. Expression of PAL31 resulted in the marked rescue of Rat1 cells from etoposide and UV radiation-induced apoptosis and the cytoprotection was correlated with the levels of PAL31 protein. Thus, cytoprotection from apoptosis is a physiological function of PAL31. PAL31 can suppress caspase-3 activity but not cytochrome c release in vitro, indicating that PAL31 is a direct caspase-3 inhibitor. In conclusion, PAL31 is a multifunctional protein working as a cell cycle progression factor as well as a cell survival factor.

Original languageEnglish
Pages (from-to)817-823
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume342
Issue number3
DOIs
Publication statusPublished - 2006 Apr 14
Externally publishedYes

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Keywords

  • Anp32b
  • Apoptosis
  • Caspase-3
  • IAP
  • LANP
  • PP2A
  • Proliferation related acidic leucine-rich protein PAL31

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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