Abstract
Skeletal muscle may develop adaptive molecular chaperone enhancements as a potential defense system through repeated daily exercise stimulation. The present study investigated whether prolonged exercise training alters the expression of molecular chaperone proteins for the long term in skeletal muscle. Mature male Wistar rats were subjected for 8 wk to either a single bout of acute intermittent treadmill running (30 m/min, 5 min x 4, 5° grade) or prolonged treadmill running training (15-40 m/min, 5 min x 4, 5-7° grade). Levels of five molecular chaperone proteins [heat shock protein (HSP)25, HSP60, glucose-regulated protein (GRP)78, HSP70, and heat shock cognate (HSC)70] were measured in response to acute exercise and prolonged training. HSP70 levels were increased 6 and 24 h after acute exercise, but expression returned to control level within 2 days. In contrast, prolonged training had a long-term effect on HSP70 expression. Levels of HSP70 were notably increased by 4.5-fold over control 2 days after prolonged training; the enhancement was maintained for at least 14 days after training ended. However, other molecular chaperone proteins did not show adaptive changes in response to prolonged training. In addition, HSP70 enhancement by prolonged exercise training was not accompanied by transcription of HSP70 mRNA. These findings demonstrate that prolonged training can induce long-term enhancement of HSP70 expression without change at the mRNA level in skeletal muscle.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Regulatory Integrative and Comparative Physiology |
| Volume | 296 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2009 May |
Fingerprint
Keywords
- Adaptation
- Heat shock protein 70
- Molecular chaperone
- Skeletal muscle
ASJC Scopus subject areas
- Physiology
- Physiology (medical)
Cite this
Prolonged exercise training induces long-term enhancement of HSP70 expression in rat plantaris muscle. / Ogata, Tomonori; Oishi, Yasuharu; Higashida, Kazuhiko; Higuchi, Mitsuru; Muraoka, Isao.
In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 296, No. 5, 05.2009.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prolonged exercise training induces long-term enhancement of HSP70 expression in rat plantaris muscle
AU - Ogata, Tomonori
AU - Oishi, Yasuharu
AU - Higashida, Kazuhiko
AU - Higuchi, Mitsuru
AU - Muraoka, Isao
PY - 2009/5
Y1 - 2009/5
N2 - Skeletal muscle may develop adaptive molecular chaperone enhancements as a potential defense system through repeated daily exercise stimulation. The present study investigated whether prolonged exercise training alters the expression of molecular chaperone proteins for the long term in skeletal muscle. Mature male Wistar rats were subjected for 8 wk to either a single bout of acute intermittent treadmill running (30 m/min, 5 min x 4, 5° grade) or prolonged treadmill running training (15-40 m/min, 5 min x 4, 5-7° grade). Levels of five molecular chaperone proteins [heat shock protein (HSP)25, HSP60, glucose-regulated protein (GRP)78, HSP70, and heat shock cognate (HSC)70] were measured in response to acute exercise and prolonged training. HSP70 levels were increased 6 and 24 h after acute exercise, but expression returned to control level within 2 days. In contrast, prolonged training had a long-term effect on HSP70 expression. Levels of HSP70 were notably increased by 4.5-fold over control 2 days after prolonged training; the enhancement was maintained for at least 14 days after training ended. However, other molecular chaperone proteins did not show adaptive changes in response to prolonged training. In addition, HSP70 enhancement by prolonged exercise training was not accompanied by transcription of HSP70 mRNA. These findings demonstrate that prolonged training can induce long-term enhancement of HSP70 expression without change at the mRNA level in skeletal muscle.
AB - Skeletal muscle may develop adaptive molecular chaperone enhancements as a potential defense system through repeated daily exercise stimulation. The present study investigated whether prolonged exercise training alters the expression of molecular chaperone proteins for the long term in skeletal muscle. Mature male Wistar rats were subjected for 8 wk to either a single bout of acute intermittent treadmill running (30 m/min, 5 min x 4, 5° grade) or prolonged treadmill running training (15-40 m/min, 5 min x 4, 5-7° grade). Levels of five molecular chaperone proteins [heat shock protein (HSP)25, HSP60, glucose-regulated protein (GRP)78, HSP70, and heat shock cognate (HSC)70] were measured in response to acute exercise and prolonged training. HSP70 levels were increased 6 and 24 h after acute exercise, but expression returned to control level within 2 days. In contrast, prolonged training had a long-term effect on HSP70 expression. Levels of HSP70 were notably increased by 4.5-fold over control 2 days after prolonged training; the enhancement was maintained for at least 14 days after training ended. However, other molecular chaperone proteins did not show adaptive changes in response to prolonged training. In addition, HSP70 enhancement by prolonged exercise training was not accompanied by transcription of HSP70 mRNA. These findings demonstrate that prolonged training can induce long-term enhancement of HSP70 expression without change at the mRNA level in skeletal muscle.
KW - Adaptation
KW - Heat shock protein 70
KW - Molecular chaperone
KW - Skeletal muscle
UR - http://www.scopus.com/inward/record.url?scp=66149130634&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=66149130634&partnerID=8YFLogxK
U2 - 10.1152/ajpregu.90911.2008
DO - 10.1152/ajpregu.90911.2008
M3 - Article
C2 - 19244585
AN - SCOPUS:66149130634
VL - 296
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0193-1849
IS - 5
ER -