Prolonged exercise training induces long-term enhancement of HSP70 expression in rat plantaris muscle

Tomonori Ogata, Yasuharu Oishi, Kazuhiko Higashida, Mitsuru Higuchi, Isao Muraoka

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Skeletal muscle may develop adaptive molecular chaperone enhancements as a potential defense system through repeated daily exercise stimulation. The present study investigated whether prolonged exercise training alters the expression of molecular chaperone proteins for the long term in skeletal muscle. Mature male Wistar rats were subjected for 8 wk to either a single bout of acute intermittent treadmill running (30 m/min, 5 min x 4, 5° grade) or prolonged treadmill running training (15-40 m/min, 5 min x 4, 5-7° grade). Levels of five molecular chaperone proteins [heat shock protein (HSP)25, HSP60, glucose-regulated protein (GRP)78, HSP70, and heat shock cognate (HSC)70] were measured in response to acute exercise and prolonged training. HSP70 levels were increased 6 and 24 h after acute exercise, but expression returned to control level within 2 days. In contrast, prolonged training had a long-term effect on HSP70 expression. Levels of HSP70 were notably increased by 4.5-fold over control 2 days after prolonged training; the enhancement was maintained for at least 14 days after training ended. However, other molecular chaperone proteins did not show adaptive changes in response to prolonged training. In addition, HSP70 enhancement by prolonged exercise training was not accompanied by transcription of HSP70 mRNA. These findings demonstrate that prolonged training can induce long-term enhancement of HSP70 expression without change at the mRNA level in skeletal muscle.

Original languageEnglish
Pages (from-to)R1557-R1563
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume296
Issue number5
DOIs
Publication statusPublished - 2009 May 1

Keywords

  • Adaptation
  • Heat shock protein 70
  • Molecular chaperone
  • Skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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