Prolonged Oxygen-Carrying Ability of Hemoglobin Vesicles by Coencapsulation of Catalase in Vivo

Yuji Teramura, Hideo Kanazawa, Hiromi Sakai, Shinji Takeoka, Eishun Tsuchida

    Research output: Contribution to journalArticle

    32 Citations (Scopus)

    Abstract

    Hemoglobin (Hb) vesicles (particle diameter, ca. 250 nm) have been developed as Hb-based oxygen carriers in which a purified Hb solution is encapsulated with a phospholipid bilayer membrane. The oxidation of Hb to nonfunctional ferric Hb (metHb) was caused by reactive oxygen species, especially hydrogen peroxide (H2O2), in vivo in addition to autoxidation. We focused on the enzymatic elimination of H2O 2 to suppress the metHb formation in the Hb vesicles. In this study, we coencapsulated catalase with Hb within vesicles and studied the rate of metHb formation in vivo. The Hb vesicles containing 5.6 x 104 unit mL-1 catalase decreased the rate of metHb formation by half in comparison with Hb vesicles without catalase. We succeeded in prolonging the oxygen-carrying ability of the Hb vesicle in vivo by the coencapsulation of catalase.

    Original languageEnglish
    Pages (from-to)1171-1176
    Number of pages6
    JournalBioconjugate Chemistry
    Volume14
    Issue number6
    DOIs
    Publication statusPublished - 2003 Nov

    Fingerprint

    Hemoglobin
    Catalase
    Hemoglobins
    Oxygen
    Phospholipids
    Hydrogen peroxide
    Hydrogen Peroxide
    Reactive Oxygen Species
    Membranes
    Oxidation

    ASJC Scopus subject areas

    • Chemistry(all)
    • Organic Chemistry
    • Clinical Biochemistry
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry

    Cite this

    Prolonged Oxygen-Carrying Ability of Hemoglobin Vesicles by Coencapsulation of Catalase in Vivo. / Teramura, Yuji; Kanazawa, Hideo; Sakai, Hiromi; Takeoka, Shinji; Tsuchida, Eishun.

    In: Bioconjugate Chemistry, Vol. 14, No. 6, 11.2003, p. 1171-1176.

    Research output: Contribution to journalArticle

    Teramura, Yuji ; Kanazawa, Hideo ; Sakai, Hiromi ; Takeoka, Shinji ; Tsuchida, Eishun. / Prolonged Oxygen-Carrying Ability of Hemoglobin Vesicles by Coencapsulation of Catalase in Vivo. In: Bioconjugate Chemistry. 2003 ; Vol. 14, No. 6. pp. 1171-1176.
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    AU - Takeoka, Shinji

    AU - Tsuchida, Eishun

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