Prolonged Oxygen-Carrying Ability of Hemoglobin Vesicles by Coencapsulation of Catalase in Vivo

Yuji Teramura, Hideo Kanazawa, Hiromi Sakai, Shinji Takeoka, Eishun Tsuchida

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Hemoglobin (Hb) vesicles (particle diameter, ca. 250 nm) have been developed as Hb-based oxygen carriers in which a purified Hb solution is encapsulated with a phospholipid bilayer membrane. The oxidation of Hb to nonfunctional ferric Hb (metHb) was caused by reactive oxygen species, especially hydrogen peroxide (H2O2), in vivo in addition to autoxidation. We focused on the enzymatic elimination of H2O 2 to suppress the metHb formation in the Hb vesicles. In this study, we coencapsulated catalase with Hb within vesicles and studied the rate of metHb formation in vivo. The Hb vesicles containing 5.6 x 104 unit mL-1 catalase decreased the rate of metHb formation by half in comparison with Hb vesicles without catalase. We succeeded in prolonging the oxygen-carrying ability of the Hb vesicle in vivo by the coencapsulation of catalase.

Original languageEnglish
Pages (from-to)1171-1176
Number of pages6
JournalBioconjugate Chemistry
Volume14
Issue number6
DOIs
Publication statusPublished - 2003 Nov 1

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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