Promoter activity and chromosomal location of the Rana rugosa P450 aromatase (CYP19) gene

Yuki Oshima, Takuma Kato, Deshou Wang, Takahiro Murakami, Yoichi Matsuda, Yoshitaka Nagahama, Masahisa Nakamura

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Sex is determined genetically in amphibians, but is reversed occasionally by steroid hormones. The phenotypic sex of some amphibian species can be reversed from male to female by estrogens. Estrogens, which are synthesized from testosterone irreversibly by the enzyme P450 aromatase (CYP19), are essential for ovarian development in vertebrates. CYP19 expression is reportedly regulated by steroidogenic factor-1 (SF-1), also designated as Ad4BP, in fish and mammals. However, it is unknown if this is also the case in amphibians. Thus, to elucidate the role of SF-1 in CYP19 gene expression in the gonad of amphibians, it is necessary to isolate and characterize the promoter region of the CYP19 gene of amphibians. For this purpose, we first cloned the promoter region of CYP19 from genomic DNA fragments of the frog Rana rugosa. As a result, a potential binding site of SF-1 was found in the region. When a luciferase promoter assay in HEK 293 cells was carried out to examine the ability of SF-1 as a transcriptional regulator, we found that R. rugosa SF-1 stimulated the expression of the CYP19 gene of the tilapia Oreochromis niloticus, but not that of the frogs R. rugosa and Xenopus laevis. RT-PCR analysis revealed that CYP19 mRNA was expressed at a higher level in the indifferent gonads of females than in those of males. This was also true to SF-1 mRNA In addition, FISH analysis showed that the CYP19 gene was located on chromosome 3 of R. rugosa. Taken together, our data suggest that CYP19, an autosomal gene, is expressed in the undifferentiated gonads to an extent greater in females than in males, but its expression probably is not regulated by SF-1 alone. Another factor(s) may be required if SF-1 promotes the expression of the CYP19 gene in R. rugosa as it does in fish and mammals.

    Original languageEnglish
    Pages (from-to)79-85
    Number of pages7
    JournalZoological Science
    Volume23
    Issue number1
    DOIs
    Publication statusPublished - 2006 Jan

    Fingerprint

    amphibians
    promoter regions
    gonads
    genes
    gene expression
    estrogens
    frogs
    mammals
    ovarian development
    gender
    Xenopus laevis
    steroid hormones
    tilapia (common name)
    luciferase
    Oreochromis niloticus
    fish
    testosterone
    binding sites
    transcription factors
    reverse transcriptase polymerase chain reaction

    Keywords

    • Ad4BP/SF-1
    • CYP19
    • FISH analysis
    • Luciferase assay
    • Rana rugosa

    ASJC Scopus subject areas

    • Animal Science and Zoology

    Cite this

    Oshima, Y., Kato, T., Wang, D., Murakami, T., Matsuda, Y., Nagahama, Y., & Nakamura, M. (2006). Promoter activity and chromosomal location of the Rana rugosa P450 aromatase (CYP19) gene. Zoological Science, 23(1), 79-85. https://doi.org/10.2108/zsj.23.79

    Promoter activity and chromosomal location of the Rana rugosa P450 aromatase (CYP19) gene. / Oshima, Yuki; Kato, Takuma; Wang, Deshou; Murakami, Takahiro; Matsuda, Yoichi; Nagahama, Yoshitaka; Nakamura, Masahisa.

    In: Zoological Science, Vol. 23, No. 1, 01.2006, p. 79-85.

    Research output: Contribution to journalArticle

    Oshima, Y, Kato, T, Wang, D, Murakami, T, Matsuda, Y, Nagahama, Y & Nakamura, M 2006, 'Promoter activity and chromosomal location of the Rana rugosa P450 aromatase (CYP19) gene', Zoological Science, vol. 23, no. 1, pp. 79-85. https://doi.org/10.2108/zsj.23.79
    Oshima, Yuki ; Kato, Takuma ; Wang, Deshou ; Murakami, Takahiro ; Matsuda, Yoichi ; Nagahama, Yoshitaka ; Nakamura, Masahisa. / Promoter activity and chromosomal location of the Rana rugosa P450 aromatase (CYP19) gene. In: Zoological Science. 2006 ; Vol. 23, No. 1. pp. 79-85.
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