Promotion of plantlet formation from somatic embryos of carrot treated with a high molecular weight extract from a marine cyanobacterium

Hitoshi Wake, Akinobu Akasaka, Hironori Umetsu, Yoshihiro Ozeki, Koichiro Shimomura, Tadashi Matsunaga

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Somatic embryos of Daucus carota L. developed into plantlets at high frequency after addition of an extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. High molecular weight, nondialyzing fraction, separated from the extract, possessed enhanced plantlet formation promoting activity. Plantlet formation frequency was 60 % after addition of nondialysate (100 mg/l) compared to 28 % without addition. Embryos treated with the nondialysate contained five times more chlorophyll than nontreated embryos after 6 days of culture. The chlorophyll a/b ratio of 4-day old treated somatic embryos was found to be similar to that of zygotic embryos. However, the chlorophyll a/b ratio of plantlets induced from nontreated somatic embryos was variable. Nondialysate was fractionated by ultracentrifugation and an active component obtained, which gave a maximum plantlet formation frequency of 71 %, and induced rapid greening of shoots.

Original languageEnglish
Pages (from-to)62-65
Number of pages4
JournalPlant Cell Reports
Volume11
Issue number2
DOIs
Publication statusPublished - 1992 Mar
Externally publishedYes

    Fingerprint

Keywords

  • Chlorophyll content
  • Daucus carota L.
  • Marine Cyanobacteria
  • Plantlet formation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this