Promotion of plantlet formation from somatic embryos of carrot treated with a high molecular weight extract from a marine cyanobacterium

Hitoshi Wake, Akinobu Akasaka, Hironori Umetsu, Yoshihiro Ozeki, Koichiro Shimomura, Tadashi Matsunaga

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Somatic embryos of Daucus carota L. developed into plantlets at high frequency after addition of an extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. High molecular weight, nondialyzing fraction, separated from the extract, possessed enhanced plantlet formation promoting activity. Plantlet formation frequency was 60 % after addition of nondialysate (100 mg/l) compared to 28 % without addition. Embryos treated with the nondialysate contained five times more chlorophyll than nontreated embryos after 6 days of culture. The chlorophyll a/b ratio of 4-day old treated somatic embryos was found to be similar to that of zygotic embryos. However, the chlorophyll a/b ratio of plantlets induced from nontreated somatic embryos was variable. Nondialysate was fractionated by ultracentrifugation and an active component obtained, which gave a maximum plantlet formation frequency of 71 %, and induced rapid greening of shoots.

Original languageEnglish
Pages (from-to)62-65
Number of pages4
JournalPlant Cell Reports
Volume11
Issue number2
DOIs
Publication statusPublished - 1992 Mar
Externally publishedYes

Fingerprint

somatic embryos
carrots
plantlets
Cyanobacteria
molecular weight
chlorophyll
embryo (plant)
extracts
ultracentrifugation
Synechococcus
Daucus carota
shoots

Keywords

  • Chlorophyll content
  • Daucus carota L.
  • Marine Cyanobacteria
  • Plantlet formation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Promotion of plantlet formation from somatic embryos of carrot treated with a high molecular weight extract from a marine cyanobacterium. / Wake, Hitoshi; Akasaka, Akinobu; Umetsu, Hironori; Ozeki, Yoshihiro; Shimomura, Koichiro; Matsunaga, Tadashi.

In: Plant Cell Reports, Vol. 11, No. 2, 03.1992, p. 62-65.

Research output: Contribution to journalArticle

Wake, Hitoshi ; Akasaka, Akinobu ; Umetsu, Hironori ; Ozeki, Yoshihiro ; Shimomura, Koichiro ; Matsunaga, Tadashi. / Promotion of plantlet formation from somatic embryos of carrot treated with a high molecular weight extract from a marine cyanobacterium. In: Plant Cell Reports. 1992 ; Vol. 11, No. 2. pp. 62-65.
@article{612a04488c2249f39f71e81e60047c97,
title = "Promotion of plantlet formation from somatic embryos of carrot treated with a high molecular weight extract from a marine cyanobacterium",
abstract = "Somatic embryos of Daucus carota L. developed into plantlets at high frequency after addition of an extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. High molecular weight, nondialyzing fraction, separated from the extract, possessed enhanced plantlet formation promoting activity. Plantlet formation frequency was 60 {\%} after addition of nondialysate (100 mg/l) compared to 28 {\%} without addition. Embryos treated with the nondialysate contained five times more chlorophyll than nontreated embryos after 6 days of culture. The chlorophyll a/b ratio of 4-day old treated somatic embryos was found to be similar to that of zygotic embryos. However, the chlorophyll a/b ratio of plantlets induced from nontreated somatic embryos was variable. Nondialysate was fractionated by ultracentrifugation and an active component obtained, which gave a maximum plantlet formation frequency of 71 {\%}, and induced rapid greening of shoots.",
keywords = "Chlorophyll content, Daucus carota L., Marine Cyanobacteria, Plantlet formation",
author = "Hitoshi Wake and Akinobu Akasaka and Hironori Umetsu and Yoshihiro Ozeki and Koichiro Shimomura and Tadashi Matsunaga",
year = "1992",
month = "3",
doi = "10.1007/BF00235254",
language = "English",
volume = "11",
pages = "62--65",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Promotion of plantlet formation from somatic embryos of carrot treated with a high molecular weight extract from a marine cyanobacterium

AU - Wake, Hitoshi

AU - Akasaka, Akinobu

AU - Umetsu, Hironori

AU - Ozeki, Yoshihiro

AU - Shimomura, Koichiro

AU - Matsunaga, Tadashi

PY - 1992/3

Y1 - 1992/3

N2 - Somatic embryos of Daucus carota L. developed into plantlets at high frequency after addition of an extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. High molecular weight, nondialyzing fraction, separated from the extract, possessed enhanced plantlet formation promoting activity. Plantlet formation frequency was 60 % after addition of nondialysate (100 mg/l) compared to 28 % without addition. Embryos treated with the nondialysate contained five times more chlorophyll than nontreated embryos after 6 days of culture. The chlorophyll a/b ratio of 4-day old treated somatic embryos was found to be similar to that of zygotic embryos. However, the chlorophyll a/b ratio of plantlets induced from nontreated somatic embryos was variable. Nondialysate was fractionated by ultracentrifugation and an active component obtained, which gave a maximum plantlet formation frequency of 71 %, and induced rapid greening of shoots.

AB - Somatic embryos of Daucus carota L. developed into plantlets at high frequency after addition of an extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. High molecular weight, nondialyzing fraction, separated from the extract, possessed enhanced plantlet formation promoting activity. Plantlet formation frequency was 60 % after addition of nondialysate (100 mg/l) compared to 28 % without addition. Embryos treated with the nondialysate contained five times more chlorophyll than nontreated embryos after 6 days of culture. The chlorophyll a/b ratio of 4-day old treated somatic embryos was found to be similar to that of zygotic embryos. However, the chlorophyll a/b ratio of plantlets induced from nontreated somatic embryos was variable. Nondialysate was fractionated by ultracentrifugation and an active component obtained, which gave a maximum plantlet formation frequency of 71 %, and induced rapid greening of shoots.

KW - Chlorophyll content

KW - Daucus carota L.

KW - Marine Cyanobacteria

KW - Plantlet formation

UR - http://www.scopus.com/inward/record.url?scp=0003096343&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0003096343&partnerID=8YFLogxK

U2 - 10.1007/BF00235254

DO - 10.1007/BF00235254

M3 - Article

C2 - 24213486

AN - SCOPUS:0003096343

VL - 11

SP - 62

EP - 65

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 2

ER -