Propeptide of Bacillus subtilis amylase enhances extracellular production of human interferon-α in Bacillus subtilis

Hiroshi Kakeshita, Yasushi Kageyama, Katsutoshi Ara, Katsuya Ozaki, Kouji Nakamura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The Gram-positive bacterium, Bacillus subtilis and related species are widely used industrially as hosts for producing enzymes. These species possess a high potential to produce secreted proteins into the culture medium. Nevertheless, the secretion of heterologous proteins by these species is frequently inefficient. In this study, the human interferon-α2b (hIFN-α2b) was used as a heterologous model protein, to investigate the effect of B. subtilis AmyE propeptide in enhancing the secretion of heterologous proteins in B. subtilis. We found that the secretion production and activity of hIFN-α2b with AmyE propeptide increased by more than threefold, compared to that without AmyE propeptide. The maximum amount of secreted hIFN-α2b with propeptide was 14.8 ± 0.6 μg ml-1. In addition, the pro-hIFN-α2b bioactivity reached 5.4 ± 0.5 × 107 U mg-1, which is roughly the same level as that of the non-propeptide hIFN-α2b. These results indicated that AmyE propeptide enhanced the secretion of the hIFN-α2b protein from B. subtilis. This study provides a useful method to enhance the extracellular production of heterologous proteins in B. subtilis.

Original languageEnglish
Pages (from-to)1509-1517
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume89
Issue number5
DOIs
Publication statusPublished - 2011 Mar 1

Keywords

  • Bacillus subtilis
  • Heterologous proteins
  • Human interferon α
  • Propeptide
  • Protein secretion
  • Signal peptide

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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