Proteasomal degradation resolves competition between cell polarization and cellular wound healing

Keiko Kono, Yasushi Saeki, Satoshi Yoshida, Keiji Tanaka, David Pellman

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Cellular wound healing, enabling the repair of membrane damage, is ubiquitous in eukaryotes. One aspect of the wound healing response is the redirection of a polarized cytoskeleton and the secretory machinery to the damage site. Although there has been recent progress in identifying conserved proteins involved in wound healing, the mechanisms linking these components into a coherent response are not defined. Using laser damage in budding yeast, we demonstrate that local cell wall/membrane damage triggers the dispersal of proteins from the site of polarized growth, enabling their accumulation at the wound. We define a protein-kinase-C-dependent mechanism that mediates the destruction of the formin Bni1 and the exocyst component Sec3. This degradation is essential to prevent competition between the site of polarized growth and the wound. Mechanisms to overcome competition from a pre-existing polarized cytoskeleton may be a general feature of effective wound healing in polarized cells.

Original languageEnglish
Pages (from-to)151-164
Number of pages14
JournalCell
Volume150
Issue number1
DOIs
Publication statusPublished - 2012 Jul 6
Externally publishedYes

Fingerprint

Wound Healing
Polarization
Membranes
Degradation
Laser damage
Cytoskeleton
Yeast
Protein Kinase C
Machinery
Proteins
Repair
Cells
Saccharomycetales
Wounds and Injuries
Growth
Eukaryota
Cell Wall
Lasers
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Proteasomal degradation resolves competition between cell polarization and cellular wound healing. / Kono, Keiko; Saeki, Yasushi; Yoshida, Satoshi; Tanaka, Keiji; Pellman, David.

In: Cell, Vol. 150, No. 1, 06.07.2012, p. 151-164.

Research output: Contribution to journalArticle

Kono, Keiko ; Saeki, Yasushi ; Yoshida, Satoshi ; Tanaka, Keiji ; Pellman, David. / Proteasomal degradation resolves competition between cell polarization and cellular wound healing. In: Cell. 2012 ; Vol. 150, No. 1. pp. 151-164.
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