Protein 4.1N is required for translocation of inositol 1,4,5-trisphosphate receptor type 1 to the basolateral membrane domain in polarized Madin-Darby canine kidney cells

Songbai Zhang, Akihiro Mizutani, Chihiro Hisatsune, Takayasu Higo, Hiroko Bannai, Tomohiro Nakayama, Mitsuharu Hattori, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Protein 4.1N was identified as a binding molecule for the C-terminal cytoplasmic tail of inositol 1,4,5-trisphosphate receptor type 1 (IP3R1) using a yeast two-hybrid system. 4.1N and IP3R1 associate in both subconfluent and confluent Madin-Darby canine kidney (MDCK) cells, a well studied tight polarized epithelial cell line. In subconfluent MDCK cells, 4.1N is distributed in the cytoplasm and the nucleus; IP3R1 is localized in the cytoplasm. In confluent MDCK cells, both 4.1N and IP3R1 are predominantly translocated to the basolateral membrane domain, whereas 4.1R, the prototypical homologue of 4.1N, is localized at the tight junctions (Mattagajasingh, S. N., Huang, S. C., Hartenstein, J. S., and Benz, E. J., Jr. (2000) J. Biol. Chem. 275, 30573-30585), and other endoplasmic reticulum marker proteins are still present in the cytoplasm. Moreover, the 4.1N-binding region of IP3R1 is necessary and sufficient for the localization of IP3R1 at the basolateral membrane domain. A fragment of the IP3R1-binding region of 4.1N blocks the localization of co-expressed IP3R1 at the basolateral membrane domain. These data indicate that 4.1N is required for IP3R1 translocation to the basolateral membrane domain in polarized MDCK cells.

Original languageEnglish
Pages (from-to)4048-4056
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number6
DOIs
Publication statusPublished - 2003 Feb 7
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Protein 4.1N is required for translocation of inositol 1,4,5-trisphosphate receptor type 1 to the basolateral membrane domain in polarized Madin-Darby canine kidney cells'. Together they form a unique fingerprint.

  • Cite this