Protein adsorption characteristics of porous and tentacle anion-exchange membrane prepared by radiation-induced graft polymerization

Satoshi Tsuneda, Kyoichi Saito, Takanobu Sugo, Keizo Makuuchi

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A polymer chain containing a diethylamino group was grafted onto the pore surface of a porous hollow-fiber membrane by radiation-induced graft polymerization. Dependence of the protein binding capacity of the membrane on environmental parameters such as salt concentration, pH and temperature was investigated. Saturation capacity of protein bound onto the graft chain containing ion-exchange group was governed by the conformation of the graft chain and the intensity of ion-exchange interaction. The conformation of the graft chain was investigated based on the pore radius of the membrane estimated from the permeation flux of a buffer solution through the membrane. By sufficiently permeating a bovine serum albumin (BSA) solution within the concentration range of 0.2-10 mg-BSA/ml through the membrane, the BSA binding capacity was determined. With increasing salt concentration or pH of the protein buffer solution, the graft chain shrank and BSA binding capacity decreased. On the other hand, the BSA binding capacity slightly increased with increasing temperature, and the conformation of the graft chain was insensitive to temperature in the range from 278 to 303 K. The bound BSA could be quantitatively eluted by permeating a buffer solution containing 0.5 M NaCl, and no deterioration in the BSA binding capacity was observed during five cycles of adsorption, elution and conditioning.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalRadiation Physics and Chemistry
Volume46
Issue number2
DOIs
Publication statusPublished - 1995
Externally publishedYes

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albumins
serums
polymerization
anions
membranes
proteins
adsorption
radiation
permeating
buffers
salts
porosity
elution
conditioning
deterioration
temperature
hollow
ions
saturation
cycles

ASJC Scopus subject areas

  • Radiation

Cite this

Protein adsorption characteristics of porous and tentacle anion-exchange membrane prepared by radiation-induced graft polymerization. / Tsuneda, Satoshi; Saito, Kyoichi; Sugo, Takanobu; Makuuchi, Keizo.

In: Radiation Physics and Chemistry, Vol. 46, No. 2, 1995, p. 239-245.

Research output: Contribution to journalArticle

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