Protein-binding characteristics of anion-exchange particles prepared by radiation-induced graft polymerization at low temperatures

Yuichi Shimoda, Yuta Sekiya, Daisuke Umeno, Kyoichi Saito, Goro Furumoto, Hironobu Shirataki, Naohiro Shinohara, Noboru Kubota

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

An epoxy-group-containing vinyl monomer, i.e., glycidyl methacrylate, was graft-polymerized onto polyethylene particles with an average diameter of 35 μm at various reaction temperatures within 278-333 K. The produced epoxy group was converted into a diethylamino group as an anion-exchange group. From the equilibrium binding capacity of the resultant particle-packed bed for bovine serum albumin (BSA) and the pressure loss required for a protein solution to ow through the bed, the formation of graft chains that were sufficiently long to hold BSA in multilayers and allow convective flow among them was predicted. The achieved ideal adsorption characteristics enabled a higher flow rate of the protein solution, which leads to a higher overall adsorption rate of the protein because of convective flow among the graft chains.

Original languageEnglish
Pages (from-to)588-592
Number of pages5
JournalJOURNAL OF CHEMICAL ENGINEERING OF JAPAN
Volume46
Issue number9
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Anion exchange
  • Glycidyl methacrylate
  • Particles
  • Protein adsorption
  • Radiation-induced graft polymerization

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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