Protein detection using oligonucleotide probes.

Aya Shibata, Hiroshi Abe, Kazuhiro Furukawa, Satoshi Tsuneda, Yoshihiro Ito

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We developed a new nucleic acid-based fluorescence probe for protein detection. The method is based on the scission of an aptamer into two probes, which are then attached with a chemically reactive fluorogenic compound. The protein-dependent association of the two probes accelerates a chemical reaction and indicates the presence of the target protein, which is detected using a fluorescence readout. The arginine-rich motif peptide was targeted by this type of probe. In presence of the peptide, the fluorescence signal at 450 nm increased, and no significant increase in fluorescence was observed in the absence of the peptide. An oligonucleotide-based fluorescence probe was successfully applied to the detection of the ARM peptide in solution.

Original languageEnglish
Pages (from-to)157-158
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number53
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Oligonucleotide Probes
Fluorescence
Proteins
Peptides
Protein Sorting Signals
Oligonucleotides
Nucleic Acids
Arginine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Shibata, A., Abe, H., Furukawa, K., Tsuneda, S., & Ito, Y. (2009). Protein detection using oligonucleotide probes. Nucleic acids symposium series (2004), (53), 157-158.

Protein detection using oligonucleotide probes. / Shibata, Aya; Abe, Hiroshi; Furukawa, Kazuhiro; Tsuneda, Satoshi; Ito, Yoshihiro.

In: Nucleic acids symposium series (2004), No. 53, 2009, p. 157-158.

Research output: Contribution to journalArticle

Shibata, A, Abe, H, Furukawa, K, Tsuneda, S & Ito, Y 2009, 'Protein detection using oligonucleotide probes.', Nucleic acids symposium series (2004), no. 53, pp. 157-158.
Shibata, Aya ; Abe, Hiroshi ; Furukawa, Kazuhiro ; Tsuneda, Satoshi ; Ito, Yoshihiro. / Protein detection using oligonucleotide probes. In: Nucleic acids symposium series (2004). 2009 ; No. 53. pp. 157-158.
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