Protein disulfide isomerase in the acrosome of rat sperm

M. Nakamura, Y. Michikawa, T. Baba, T. Mizunaga, H. Horti, S. Okinaga, T. Kobayashi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

An endoplasmic reticulum (ER) protein, protein disulfide isomerase (PDI), was found to locate in the acrosome of rat spermatids and sperm by the avidin-biotin-peroxidasecomplex (ABC) method. Immunoblot analysis of PDI during developmental stages showed that protein was detected in the testis between the ages of 5 and 50 days. When Northern blot analysis was carried out using human PDI cDNA as a probe, a 2.6-kb PDI mRNA was found in both the spermatocytes and spermatids of rat testis. The PDI mRNA level was similar in both types of cells. It would appear that PDI may stabilize three-dimensional structures of secretory proteins in the acrosome of rat spermatids during spermiogenesis.

Original languageEnglish
Title of host publicationMolecular Andrology
Pages201-208
Number of pages8
Volume5
Edition4
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Protein Disulfide-Isomerases
Acrosome
Spermatozoa
Spermatids
Testis
Messenger RNA
Proteins
Spermatocytes
Avidin
Spermatogenesis
Biotin
Endoplasmic Reticulum
Northern Blotting
Complementary DNA

ASJC Scopus subject areas

  • Endocrinology
  • Nephrology

Cite this

Nakamura, M., Michikawa, Y., Baba, T., Mizunaga, T., Horti, H., Okinaga, S., & Kobayashi, T. (1993). Protein disulfide isomerase in the acrosome of rat sperm. In Molecular Andrology (4 ed., Vol. 5, pp. 201-208)

Protein disulfide isomerase in the acrosome of rat sperm. / Nakamura, M.; Michikawa, Y.; Baba, T.; Mizunaga, T.; Horti, H.; Okinaga, S.; Kobayashi, T.

Molecular Andrology. Vol. 5 4. ed. 1993. p. 201-208.

Research output: Chapter in Book/Report/Conference proceedingChapter

Nakamura, M, Michikawa, Y, Baba, T, Mizunaga, T, Horti, H, Okinaga, S & Kobayashi, T 1993, Protein disulfide isomerase in the acrosome of rat sperm. in Molecular Andrology. 4 edn, vol. 5, pp. 201-208.
Nakamura M, Michikawa Y, Baba T, Mizunaga T, Horti H, Okinaga S et al. Protein disulfide isomerase in the acrosome of rat sperm. In Molecular Andrology. 4 ed. Vol. 5. 1993. p. 201-208
Nakamura, M. ; Michikawa, Y. ; Baba, T. ; Mizunaga, T. ; Horti, H. ; Okinaga, S. ; Kobayashi, T. / Protein disulfide isomerase in the acrosome of rat sperm. Molecular Andrology. Vol. 5 4. ed. 1993. pp. 201-208
@inbook{eaebfe46fbe0493c96f5a519bc6df271,
title = "Protein disulfide isomerase in the acrosome of rat sperm",
abstract = "An endoplasmic reticulum (ER) protein, protein disulfide isomerase (PDI), was found to locate in the acrosome of rat spermatids and sperm by the avidin-biotin-peroxidasecomplex (ABC) method. Immunoblot analysis of PDI during developmental stages showed that protein was detected in the testis between the ages of 5 and 50 days. When Northern blot analysis was carried out using human PDI cDNA as a probe, a 2.6-kb PDI mRNA was found in both the spermatocytes and spermatids of rat testis. The PDI mRNA level was similar in both types of cells. It would appear that PDI may stabilize three-dimensional structures of secretory proteins in the acrosome of rat spermatids during spermiogenesis.",
author = "M. Nakamura and Y. Michikawa and T. Baba and T. Mizunaga and H. Horti and S. Okinaga and T. Kobayashi",
year = "1993",
language = "English",
volume = "5",
pages = "201--208",
booktitle = "Molecular Andrology",
edition = "4",

}

TY - CHAP

T1 - Protein disulfide isomerase in the acrosome of rat sperm

AU - Nakamura, M.

AU - Michikawa, Y.

AU - Baba, T.

AU - Mizunaga, T.

AU - Horti, H.

AU - Okinaga, S.

AU - Kobayashi, T.

PY - 1993

Y1 - 1993

N2 - An endoplasmic reticulum (ER) protein, protein disulfide isomerase (PDI), was found to locate in the acrosome of rat spermatids and sperm by the avidin-biotin-peroxidasecomplex (ABC) method. Immunoblot analysis of PDI during developmental stages showed that protein was detected in the testis between the ages of 5 and 50 days. When Northern blot analysis was carried out using human PDI cDNA as a probe, a 2.6-kb PDI mRNA was found in both the spermatocytes and spermatids of rat testis. The PDI mRNA level was similar in both types of cells. It would appear that PDI may stabilize three-dimensional structures of secretory proteins in the acrosome of rat spermatids during spermiogenesis.

AB - An endoplasmic reticulum (ER) protein, protein disulfide isomerase (PDI), was found to locate in the acrosome of rat spermatids and sperm by the avidin-biotin-peroxidasecomplex (ABC) method. Immunoblot analysis of PDI during developmental stages showed that protein was detected in the testis between the ages of 5 and 50 days. When Northern blot analysis was carried out using human PDI cDNA as a probe, a 2.6-kb PDI mRNA was found in both the spermatocytes and spermatids of rat testis. The PDI mRNA level was similar in both types of cells. It would appear that PDI may stabilize three-dimensional structures of secretory proteins in the acrosome of rat spermatids during spermiogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0027861077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027861077&partnerID=8YFLogxK

M3 - Chapter

AN - SCOPUS:0027861077

VL - 5

SP - 201

EP - 208

BT - Molecular Andrology

ER -