Protein kinase C and c-myc gene activation pathways in thrombopoietin signal transduction

Masae Kunitama, Ritsuko Shimizu, Minami Yamada, Takashi Kato, Hiroshi Miyazaki, Koji Okada, Yasusada Miura, Norio Komatsu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Thrombopoietin (TPO) is the major regulator of the proliferation and differentiation of megakaryocyte precursors through interaction with its receptor encoded by the c-mpl protooncogene. We established the human TPO-dependent leukemia cell line, UT-7/TPO. In these cells, TPO activated protein kinase C (PKC) in a time dependent manner. Subsequently, the c-myc gene was transiently induced to a maximal level 60-90 minutes after TPO exposure. In addition, we found that stimulating UT-7/TPO cells with TPO rapidly induces the significant accumulation of inositol 1,4,5-trisphosphate (Ins-P3), leading to the mobilization of calcium from intracellular stores. Taken together, the activation of PKC and subsequent c-myc gene induction are involved in the TPO-induced cellular response(s), presumably through the activation of PLC.

Original languageEnglish
Pages (from-to)290-294
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume231
Issue number2
DOIs
Publication statusPublished - 1997 Feb 13
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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