Proteins found in a CikA interaction assay link the orcadian clock, metabolism, and cell division in synechococcus elongatus

Shannon R. Mackey, Jong Soon Choi, Yohko Kitayama, Hideo Iwasaki, Guogang Dong, Susan S. Golden

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    Diverse organisms time their cellular activities to occur at distinct phases of Earth's solar day, not through the direct regulation of these processes by light and darkness but rather through the use of an internal biological (circadian) clock that is synchronized with the external cycle. Input pathways serve as mechanisms to transduce external cues to a circadian oscillator to maintain synchrony between this internal oscillation and the environment. The circadian input pathway in the cyanobacterium Synechococcus elongatus PCC 7942 requires the kinase CikA. A cikA null mutant exhibits a short circadian period, the inability to reset its clock in response to pulses of darkness, and a defect in cell division. Although CikA is copurified with the Kai proteins that constitute the circadian central oscillator, no direct interaction between CikA and either KaiA, KaiB, or KaiC has been demonstrated. Here, we identify four proteins that may help connect CikA with the oscillator. Phenotypic analyses of null and overexpression alleles demonstrate that these proteins are involved in at least one of the functions-circadian period regulation, phase resetting, and cell division-attributed to CikA. Predictions based on sequence similarity suggest that these proteins function through protein phosphorylation, iron-sulfur cluster biosynthesis, and redox regulation. Collectively, these results suggest a model for circadian input that incorporates proteins that link the circadian clock, metabolism, and cell division.

    Original languageEnglish
    Pages (from-to)3738-3746
    Number of pages9
    JournalJournal of Bacteriology
    Volume190
    Issue number10
    DOIs
    Publication statusPublished - 2008 May

    Fingerprint

    Synechococcus
    Cell Division
    Circadian Clocks
    Darkness
    Proteins
    Iron-Sulfur Proteins
    Biological Clocks
    Cyanobacteria
    Oxidation-Reduction
    Cues
    Phosphotransferases
    Alleles
    Phosphorylation
    Light

    ASJC Scopus subject areas

    • Microbiology
    • Molecular Biology
    • Applied Microbiology and Biotechnology
    • Immunology

    Cite this

    Proteins found in a CikA interaction assay link the orcadian clock, metabolism, and cell division in synechococcus elongatus. / Mackey, Shannon R.; Choi, Jong Soon; Kitayama, Yohko; Iwasaki, Hideo; Dong, Guogang; Golden, Susan S.

    In: Journal of Bacteriology, Vol. 190, No. 10, 05.2008, p. 3738-3746.

    Research output: Contribution to journalArticle

    Mackey, Shannon R. ; Choi, Jong Soon ; Kitayama, Yohko ; Iwasaki, Hideo ; Dong, Guogang ; Golden, Susan S. / Proteins found in a CikA interaction assay link the orcadian clock, metabolism, and cell division in synechococcus elongatus. In: Journal of Bacteriology. 2008 ; Vol. 190, No. 10. pp. 3738-3746.
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