Proteomic analysis of irregular, bullet-shaped magnetosomes in the sulphate-reducing magnetotactic bacterium Desulfovibrio magneticus RS-1

Tadashi Matsunaga, Michiko Nemoto, Atsushi Arakaki, Masayoshi Tanaka

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Recent molecular studies on magnetotactic bacteria have identified a number of proteins associated with bacterial magnetites (magnetosomes) and elucidated their importance in magnetite biomineralisation. However, these analyses were limited to magnetotactic bacterial strains belonging to the α-subclass of Proteobacteria. We performed a proteomic analysis of magnetosome membrane proteins in Desulfovibrio magneticus strain RS-1, which is phylogenetically classified as a member of the δ-Proteobacteria. In the analysis, the identified proteins were classified based on their putative functions and compared with the proteins from the other magnetotactic bacteria, Magnetospirillum magneticum AMB-1 and M. gryphiswaldense MSR-1. Three magnetosome-specific proteins, MamA (Mms24), MamK, and MamM, were identified in strains RS-1, AMB-1, and MSR-1. Furthermore, genes encoding ten magnetosome membrane proteins, including novel proteins, were assigned to a putative magnetosome island that contains subsets of genes essential for magnetosome formation. The collagen-like protein and putative iron-binding proteins, which are considered to play key roles in magnetite crystal formation, were identified as specific proteins in strain RS-1. Furthermore, genes encoding two homologous proteins of Magnetococcus MC-1 were assigned to a cryptic plasmid of strain RS-1. The newly identified magnetosome membrane proteins might contribute to the formation of the unique irregular, bullet-shaped crystals in this microorganism.

Original languageEnglish
Pages (from-to)3341-3352
Number of pages12
JournalProteomics
Volume9
Issue number12
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Magnetosomes
Desulfovibrio
Proteomics
Sulfates
Bacteria
Ferrosoferric Oxide
Proteins
Proteobacteria
Membrane Proteins
Gene encoding
Magnetospirillum
Iron-Binding Proteins
Biomineralization
Crystals
Essential Genes
Microorganisms
Genes
Plasmids
Collagen

Keywords

  • Biomineralisation
  • Desulfovibrio
  • Magnetosome
  • Magnetotactic bacteria

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Medicine(all)

Cite this

Proteomic analysis of irregular, bullet-shaped magnetosomes in the sulphate-reducing magnetotactic bacterium Desulfovibrio magneticus RS-1. / Matsunaga, Tadashi; Nemoto, Michiko; Arakaki, Atsushi; Tanaka, Masayoshi.

In: Proteomics, Vol. 9, No. 12, 2009, p. 3341-3352.

Research output: Contribution to journalArticle

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