Protocatechuate 3,4-dioxygenase from nocardia erythropolis

Ryuichiro Kurane, Katsutoshi Ara, Isei Nakamura, Tomoo Suzuki, Seiichi Fukuoka

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Protocatechuate 3,4-dioxygenase was isolated from a gram-positive bacterium, Nocardia erythropolis, the enzyme participates in the phthalate ester metabolism in the bacterium. Cultural conditions for production of the enzyme, the purification procedure, and some properties of the enzyme were studied. A bouillon (beef) medium was the most effective among those tested for cell growth and enzyme formation. The effect was due to the ring closure type of creatine compounds. Protocatechuate 3,4-dioxygenase was purified from the cell-free extract ca. 1,400-fold and it gave a single band on polyacrylamide gel electrophoresis. The molecular weight was estimated to be ca. 150,000. The optimal pH and temperature were pH 8.0 and 40°C, respectively. The enzyme was stable in a pH range from 7.6 to 8.6 and below 42°C. The enzyme was inhibited by several metals such as Pb2+, Cd2+and Hg2+. The enzyme was active on a wide range of o-dihydroxyphenyl compounds, in contrast to the high specificity of similar enzymes from gram-negative bacteria (Pseudomonas). The enzyme had a broad absorption band in the visible region with a peak around 450 nm, suggesting the presence of non-heme ion(s) bound to the enzyme as a cofactor. The spectrum changed markedly upon addition of the substrate, possibly showing the formation of an enzyme-substrate complex.

Original languageEnglish
Pages (from-to)2105-2111
Number of pages7
JournalAgricultural and Biological Chemistry
Volume48
Issue number8
DOIs
Publication statusPublished - 1984 Aug
Externally publishedYes

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Protocatechuate-3,4-Dioxygenase
Nocardia
Enzymes
enzymes
Bacteria
phthalates
creatine
Beef
enzyme substrates
Creatine
Gram-positive bacteria
Cell growth
Gram-Positive Bacteria
Gram-negative bacteria
Substrates
Pseudomonas
Cell Extracts
Electrophoresis
Gram-Negative Bacteria
polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Protocatechuate 3,4-dioxygenase from nocardia erythropolis. / Kurane, Ryuichiro; Ara, Katsutoshi; Nakamura, Isei; Suzuki, Tomoo; Fukuoka, Seiichi.

In: Agricultural and Biological Chemistry, Vol. 48, No. 8, 08.1984, p. 2105-2111.

Research output: Contribution to journalArticle

Kurane, Ryuichiro ; Ara, Katsutoshi ; Nakamura, Isei ; Suzuki, Tomoo ; Fukuoka, Seiichi. / Protocatechuate 3,4-dioxygenase from nocardia erythropolis. In: Agricultural and Biological Chemistry. 1984 ; Vol. 48, No. 8. pp. 2105-2111.
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