Proton mediated control of biochemical reactions with bioelectronic pH modulation

Yingxin Deng, Takeo Miyake, Scott Keene, Erik E. Josberger, Marco Rolandi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In Nature, protons (H+) can mediate metabolic process through enzymatic reactions. Examples include glucose oxidation with glucose dehydrogenase to regulate blood glucose level, alcohol dissolution into carboxylic acid through alcohol dehydrogenase, and voltage-regulated H+ channels activating bioluminescence in firefly and jellyfish. Artificial devices that control H+ currents and H+ concentration (pH) are able to actively influence biochemical processes. Here, we demonstrate a biotransducer that monitors and actively regulates pH-responsive enzymatic reactions by monitoring and controlling the flow of H+ between PdHx contacts and solution. The present transducer records bistable pH modulation from an "enzymatic flip-flop" circuit that comprises glucose dehydrogenase and alcohol dehydrogenase. The transducer also controls bioluminescence from firefly luciferase by affecting solution pH.

Original languageEnglish
Article number24080
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 Apr 7
Externally publishedYes

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Protons
Glucose 1-Dehydrogenase
Alcohol Dehydrogenase
Transducers
Biochemical Phenomena
Fireflies
Firefly Luciferases
Carboxylic Acids
Blood Glucose
Alcohols
Glucose
Equipment and Supplies

ASJC Scopus subject areas

  • General

Cite this

Proton mediated control of biochemical reactions with bioelectronic pH modulation. / Deng, Yingxin; Miyake, Takeo; Keene, Scott; Josberger, Erik E.; Rolandi, Marco.

In: Scientific Reports, Vol. 6, 24080, 07.04.2016.

Research output: Contribution to journalArticle

Deng, Yingxin ; Miyake, Takeo ; Keene, Scott ; Josberger, Erik E. ; Rolandi, Marco. / Proton mediated control of biochemical reactions with bioelectronic pH modulation. In: Scientific Reports. 2016 ; Vol. 6.
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