Protonatable ionenes for nucleic acid complexation

Sean M. Ramirez, John M. Layman, Timothy Edward Long

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Novel tboc-protected ionenes with M̄w exceeding 30 kDa were prepared from the step-growth polymerization of tert-butyl bis[3- (dimethylamino)propyl]carbamate and 1,12-dibromododecane. The protected ionenes yielded pH-sensitive, protonatable ionenes with pKa≈6.6 for the conjugate acid of the protonated secondary amine. Polyplexes of the protected and deprotected ionenes, whose cytotoxicity for endothelial cells was analyzed using the MTT assay, efficiently complex plasmid DNA. Polyplexes destabilized cellular membranes as revealed using the lactate dehydrogenase assay at high concentrations. The polyplexes were successfully transfected into HBMECs at mass ratios 2, 4, 8, 12, and 16 (polymer/DNA) at polyplex concentrations less than 10 μg · mL-1. (Figure Presented)

Original languageEnglish
Pages (from-to)1127-1134
Number of pages8
JournalMacromolecular Bioscience
Volume9
Issue number11
DOIs
Publication statusPublished - 2009 Nov 10
Externally publishedYes

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Keywords

  • Biocompatibility
  • Ionenes
  • Non-viral transfection
  • Polyelectrolytes
  • Proton sponge
  • Step-growth polymerization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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