PROTOPLAST FORMATION AND REGENERATION IN LACTOBACILLUS CASEI.

Toshinori Hirano, Kotaro Kirimura, Shoji Usami

    Research output: Contribution to journalArticle

    Abstract

    The conditions for the protoplast formation and the cell wall regeneration of Lactobacillus casei IFO-3831 are described. Since L. casei was resistant to the action of lysozyme, the cell wall digestion was insufficient when the cells were treated with lysozyme at various concentrations. The protoplasts were successfully produced by the treatment with mutanolysin. Approximately 100% of protoplast forming ratio was achieved when the cell suspension was incubated in the presence of 50 unit/ml of the enzyme in 5 mM Tris-maleate-0. 09 M sucrose solution (pH 6. 2) at 37 degree C for 60 min. The burst of protoplasts and the autolysis of cells happened in the buffer solution containing mutanolysin without magnesium ion, but they were restrained by addition of 10 mM magnesium ion as a final concentration to the buffer solution. The protoplast regeneration was achieved when the samples were diluted and spread over the surface of a regeneration medium. 1. 41% of the cells retained osmotic stability. The regeneration ratio of protoplasts was 1. 8%.

    Original languageEnglish
    Pages (from-to)35-40
    Number of pages6
    JournalWaseda Daigaku Rikogaku Kenkyusho Hokoku/Bulletin of Science and Engineering Research Laboratory, Waseda University
    Issue number110
    Publication statusPublished - 1985

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    Enzymes
    Magnesium
    Cells
    Ions
    Sugar (sucrose)

    ASJC Scopus subject areas

    • Engineering(all)

    Cite this

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    title = "PROTOPLAST FORMATION AND REGENERATION IN LACTOBACILLUS CASEI.",
    abstract = "The conditions for the protoplast formation and the cell wall regeneration of Lactobacillus casei IFO-3831 are described. Since L. casei was resistant to the action of lysozyme, the cell wall digestion was insufficient when the cells were treated with lysozyme at various concentrations. The protoplasts were successfully produced by the treatment with mutanolysin. Approximately 100{\%} of protoplast forming ratio was achieved when the cell suspension was incubated in the presence of 50 unit/ml of the enzyme in 5 mM Tris-maleate-0. 09 M sucrose solution (pH 6. 2) at 37 degree C for 60 min. The burst of protoplasts and the autolysis of cells happened in the buffer solution containing mutanolysin without magnesium ion, but they were restrained by addition of 10 mM magnesium ion as a final concentration to the buffer solution. The protoplast regeneration was achieved when the samples were diluted and spread over the surface of a regeneration medium. 1. 41{\%} of the cells retained osmotic stability. The regeneration ratio of protoplasts was 1. 8{\%}.",
    author = "Toshinori Hirano and Kotaro Kirimura and Shoji Usami",
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    T1 - PROTOPLAST FORMATION AND REGENERATION IN LACTOBACILLUS CASEI.

    AU - Hirano, Toshinori

    AU - Kirimura, Kotaro

    AU - Usami, Shoji

    PY - 1985

    Y1 - 1985

    N2 - The conditions for the protoplast formation and the cell wall regeneration of Lactobacillus casei IFO-3831 are described. Since L. casei was resistant to the action of lysozyme, the cell wall digestion was insufficient when the cells were treated with lysozyme at various concentrations. The protoplasts were successfully produced by the treatment with mutanolysin. Approximately 100% of protoplast forming ratio was achieved when the cell suspension was incubated in the presence of 50 unit/ml of the enzyme in 5 mM Tris-maleate-0. 09 M sucrose solution (pH 6. 2) at 37 degree C for 60 min. The burst of protoplasts and the autolysis of cells happened in the buffer solution containing mutanolysin without magnesium ion, but they were restrained by addition of 10 mM magnesium ion as a final concentration to the buffer solution. The protoplast regeneration was achieved when the samples were diluted and spread over the surface of a regeneration medium. 1. 41% of the cells retained osmotic stability. The regeneration ratio of protoplasts was 1. 8%.

    AB - The conditions for the protoplast formation and the cell wall regeneration of Lactobacillus casei IFO-3831 are described. Since L. casei was resistant to the action of lysozyme, the cell wall digestion was insufficient when the cells were treated with lysozyme at various concentrations. The protoplasts were successfully produced by the treatment with mutanolysin. Approximately 100% of protoplast forming ratio was achieved when the cell suspension was incubated in the presence of 50 unit/ml of the enzyme in 5 mM Tris-maleate-0. 09 M sucrose solution (pH 6. 2) at 37 degree C for 60 min. The burst of protoplasts and the autolysis of cells happened in the buffer solution containing mutanolysin without magnesium ion, but they were restrained by addition of 10 mM magnesium ion as a final concentration to the buffer solution. The protoplast regeneration was achieved when the samples were diluted and spread over the surface of a regeneration medium. 1. 41% of the cells retained osmotic stability. The regeneration ratio of protoplasts was 1. 8%.

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