PROTOPLAST FORMATION FROM THE MYCELIUM OF ASPERGILLUS NIGER.

Kotaro Kirimura, Shoji Usami

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Fungal protoplasts have been used as genetic tools for protoplast fusion and transformation, cell wall synthesis and enzyme localization. Protoplast formation from the mycelium of Aspergillus niger was examined with the combined use of Zymolyase, chitinase and hemicellulase or Novozym 234. Sufficient numbers of protoplasts (1. 2-5. 2 multiplied by 10**6/ml) could be obtained with the strains tested under 120 min incubation.

    Original languageEnglish
    Pages (from-to)1-5
    Number of pages5
    JournalWaseda Daigaku Rikogaku Kenkyusho Hokoku/Bulletin of Science and Engineering Research Laboratory,
    Issue number115
    Publication statusPublished - 1986

    Fingerprint

    Aspergillus
    Fusion reactions
    Enzymes
    Cells

    ASJC Scopus subject areas

    • Engineering(all)

    Cite this

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    abstract = "Fungal protoplasts have been used as genetic tools for protoplast fusion and transformation, cell wall synthesis and enzyme localization. Protoplast formation from the mycelium of Aspergillus niger was examined with the combined use of Zymolyase, chitinase and hemicellulase or Novozym 234. Sufficient numbers of protoplasts (1. 2-5. 2 multiplied by 10**6/ml) could be obtained with the strains tested under 120 min incubation.",
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    AU - Kirimura, Kotaro

    AU - Usami, Shoji

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    Y1 - 1986

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