Prototype of micro flow cell for multi-analyte sandwich immunoassay

N. Honda, Shuichi Shoji, M. Isomura, Y. Ashihara, K. Akahori, H. Sato

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    A micro flow cell for sandwich immunoassay using Teflon (Asahi Glass Co.: CYTOP™) check valves for sample and reagent inlets is designed and fabricated. The check valves are used not only to prevent reverse flow of injected solutions but also to realize sequential injection of the sample and reagent by simple pressure control. The valve structure is designed to control the width and thickness of the sample and reagent flows at relatively small flow velocity that is useful to reduce effective sample and reagent volumes. We examined a fluorescent sandwich immunoassay for human AFP (α-fetoprotein) as a preliminary experiment and faster immunoassay within 30 min compared to the conventional one was realized in the micro flow cell.

    Original languageEnglish
    Title of host publication2001 International Microprocesses and Nanotechnology Conference, MNC 2001
    PublisherInstitute of Electrical and Electronics Engineers Inc.
    Pages24-25
    Number of pages2
    ISBN (Print)4891140178, 9784891140175
    DOIs
    Publication statusPublished - 2001
    EventInternational Microprocesses and Nanotechnology Conference, MNC 2001 - Shimane, Japan
    Duration: 2001 Oct 312001 Nov 2

    Other

    OtherInternational Microprocesses and Nanotechnology Conference, MNC 2001
    CountryJapan
    CityShimane
    Period01/10/3101/11/2

    Fingerprint

    immunoassay
    Pressure control
    Polytetrafluoroethylenes
    Immunoassay
    Flow velocity
    reagents
    prototypes
    Glass
    cells
    Experiments
    Fetal Proteins
    teflon (trademark)
    Polytetrafluoroethylene
    flow velocity
    injection
    Pressure
    Injections
    glass

    Keywords

    • Check Valve
    • Clinical Diagnostics
    • Micro Flow Cell
    • Multi-analyte Sandwich Immunoassay
    • Teflon

    ASJC Scopus subject areas

    • Biotechnology
    • Fluid Flow and Transfer Processes
    • Electrical and Electronic Engineering
    • Electronic, Optical and Magnetic Materials
    • Instrumentation

    Cite this

    Honda, N., Shoji, S., Isomura, M., Ashihara, Y., Akahori, K., & Sato, H. (2001). Prototype of micro flow cell for multi-analyte sandwich immunoassay. In 2001 International Microprocesses and Nanotechnology Conference, MNC 2001 (pp. 24-25). [984048] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/IMNC.2001.984048

    Prototype of micro flow cell for multi-analyte sandwich immunoassay. / Honda, N.; Shoji, Shuichi; Isomura, M.; Ashihara, Y.; Akahori, K.; Sato, H.

    2001 International Microprocesses and Nanotechnology Conference, MNC 2001. Institute of Electrical and Electronics Engineers Inc., 2001. p. 24-25 984048.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Honda, N, Shoji, S, Isomura, M, Ashihara, Y, Akahori, K & Sato, H 2001, Prototype of micro flow cell for multi-analyte sandwich immunoassay. in 2001 International Microprocesses and Nanotechnology Conference, MNC 2001., 984048, Institute of Electrical and Electronics Engineers Inc., pp. 24-25, International Microprocesses and Nanotechnology Conference, MNC 2001, Shimane, Japan, 01/10/31. https://doi.org/10.1109/IMNC.2001.984048
    Honda N, Shoji S, Isomura M, Ashihara Y, Akahori K, Sato H. Prototype of micro flow cell for multi-analyte sandwich immunoassay. In 2001 International Microprocesses and Nanotechnology Conference, MNC 2001. Institute of Electrical and Electronics Engineers Inc. 2001. p. 24-25. 984048 https://doi.org/10.1109/IMNC.2001.984048
    Honda, N. ; Shoji, Shuichi ; Isomura, M. ; Ashihara, Y. ; Akahori, K. ; Sato, H. / Prototype of micro flow cell for multi-analyte sandwich immunoassay. 2001 International Microprocesses and Nanotechnology Conference, MNC 2001. Institute of Electrical and Electronics Engineers Inc., 2001. pp. 24-25
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    AU - Akahori, K.

    AU - Sato, H.

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