Prototype of micro flow cell for multi-analyte sandwich immunoassay

N. Honda; Shuichi Shoji; M. Isomura; Y. Ashihara; K. Akahori; H. Sato

doi:10.1109/IMNC.2001.984048

Prototype of micro flow cell for multi-analyte sandwich immunoassay

N. Honda, Shuichi Shoji, M. Isomura, Y. Ashihara, K. Akahori, H. Sato

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

A micro flow cell for sandwich immunoassay using Teflon (Asahi Glass Co.: CYTOP™) check valves for sample and reagent inlets is designed and fabricated. The check valves are used not only to prevent reverse flow of injected solutions but also to realize sequential injection of the sample and reagent by simple pressure control. The valve structure is designed to control the width and thickness of the sample and reagent flows at relatively small flow velocity that is useful to reduce effective sample and reagent volumes. We examined a fluorescent sandwich immunoassay for human AFP (α-fetoprotein) as a preliminary experiment and faster immunoassay within 30 min compared to the conventional one was realized in the micro flow cell.

Original language	English
Title of host publication	2001 International Microprocesses and Nanotechnology Conference, MNC 2001
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	24-25
Number of pages	2
ISBN (Print)	4891140178, 9784891140175
DOIs	https://doi.org/10.1109/IMNC.2001.984048
Publication status	Published - 2001
Event	International Microprocesses and Nanotechnology Conference, MNC 2001 - Shimane, Japan Duration: 2001 Oct 31 → 2001 Nov 2

Other

Other	International Microprocesses and Nanotechnology Conference, MNC 2001
Country/Territory	Japan
City	Shimane
Period	01/10/31 → 01/11/2

Keywords

Check Valve
Clinical Diagnostics
Micro Flow Cell
Multi-analyte Sandwich Immunoassay
Teflon

ASJC Scopus subject areas

Biotechnology
Fluid Flow and Transfer Processes
Electrical and Electronic Engineering
Electronic, Optical and Magnetic Materials
Instrumentation

Access to Document

10.1109/IMNC.2001.984048

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Honda, N, Shoji, S, Isomura, M, Ashihara, Y, Akahori, K & Sato, H 2001, Prototype of micro flow cell for multi-analyte sandwich immunoassay. in 2001 International Microprocesses and Nanotechnology Conference, MNC 2001., 984048, Institute of Electrical and Electronics Engineers Inc., pp. 24-25, International Microprocesses and Nanotechnology Conference, MNC 2001, Shimane, Japan, 01/10/31. https://doi.org/10.1109/IMNC.2001.984048

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abstract = "A micro flow cell for sandwich immunoassay using Teflon (Asahi Glass Co.: CYTOP{\texttrademark}) check valves for sample and reagent inlets is designed and fabricated. The check valves are used not only to prevent reverse flow of injected solutions but also to realize sequential injection of the sample and reagent by simple pressure control. The valve structure is designed to control the width and thickness of the sample and reagent flows at relatively small flow velocity that is useful to reduce effective sample and reagent volumes. We examined a fluorescent sandwich immunoassay for human AFP (α-fetoprotein) as a preliminary experiment and faster immunoassay within 30 min compared to the conventional one was realized in the micro flow cell.",

keywords = "Check Valve, Clinical Diagnostics, Micro Flow Cell, Multi-analyte Sandwich Immunoassay, Teflon",

author = "N. Honda and Shuichi Shoji and M. Isomura and Y. Ashihara and K. Akahori and H. Sato",

year = "2001",

doi = "10.1109/IMNC.2001.984048",

language = "English",

isbn = "4891140178",

pages = "24--25",

booktitle = "2001 International Microprocesses and Nanotechnology Conference, MNC 2001",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

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TY - GEN

T1 - Prototype of micro flow cell for multi-analyte sandwich immunoassay

AU - Honda, N.

AU - Shoji, Shuichi

AU - Isomura, M.

AU - Ashihara, Y.

AU - Akahori, K.

AU - Sato, H.

PY - 2001

Y1 - 2001

N2 - A micro flow cell for sandwich immunoassay using Teflon (Asahi Glass Co.: CYTOP™) check valves for sample and reagent inlets is designed and fabricated. The check valves are used not only to prevent reverse flow of injected solutions but also to realize sequential injection of the sample and reagent by simple pressure control. The valve structure is designed to control the width and thickness of the sample and reagent flows at relatively small flow velocity that is useful to reduce effective sample and reagent volumes. We examined a fluorescent sandwich immunoassay for human AFP (α-fetoprotein) as a preliminary experiment and faster immunoassay within 30 min compared to the conventional one was realized in the micro flow cell.

AB - A micro flow cell for sandwich immunoassay using Teflon (Asahi Glass Co.: CYTOP™) check valves for sample and reagent inlets is designed and fabricated. The check valves are used not only to prevent reverse flow of injected solutions but also to realize sequential injection of the sample and reagent by simple pressure control. The valve structure is designed to control the width and thickness of the sample and reagent flows at relatively small flow velocity that is useful to reduce effective sample and reagent volumes. We examined a fluorescent sandwich immunoassay for human AFP (α-fetoprotein) as a preliminary experiment and faster immunoassay within 30 min compared to the conventional one was realized in the micro flow cell.

KW - Check Valve

KW - Clinical Diagnostics

KW - Micro Flow Cell

KW - Multi-analyte Sandwich Immunoassay

KW - Teflon

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U2 - 10.1109/IMNC.2001.984048

DO - 10.1109/IMNC.2001.984048

M3 - Conference contribution

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SN - 4891140178

SN - 9784891140175

SP - 24

EP - 25

BT - 2001 International Microprocesses and Nanotechnology Conference, MNC 2001

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - International Microprocesses and Nanotechnology Conference, MNC 2001

Y2 - 31 October 2001 through 2 November 2001

ER -

Prototype of micro flow cell for multi-analyte sandwich immunoassay

Abstract

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Keywords

ASJC Scopus subject areas

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