Psg18 is specifically expressed in follicle-associated epithelium

Kazuya Kawano, Masashi Ebisawa, Koji Hase, Shinji Fukuda, Atsushi Hijikata, Sayaka Kawano, Yasuhiro Date, Satoshi Tsuneda, Kikuji Itoh, Hiroshi Ohno

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Pregnancy-specific glycoproteins (Psgs) secreted by the placenta regulate the immune system to ensure the survival of the fetal allograft by inducing IL-10, an anti-inflammatory cytokine. However, it is unknown whether Psgs are involved in more general aspects of immune response other than maternal immunity. Here, we report that Psg18 is highly expressed in the follicle-associated epithelium (FAE) overlaying Peyer's patches (PPs). Bioinformatics analysis with Reference Database for Immune Cells (RefDIC) as well as RT-PCR data demonstrated that Psg18 is exclusively expressed in FAE in adult mice, in contrast to other Psg family members that are either not expressed or only slightly expressed in FAE. Psg18 expression was observed in FAE of germ-free-conditioned mice, and was slightly upregulated after bacterial inoculation. In situ hybridization analysis revealed that Psg18 is widely expressed throughout FAE. Furthermore, Psg18 protein is deposited on the extracellular matrix in the subepithelial dome beneath FAE, where antigen-presenting cells accumulate. These results suggest that Psg18 is an FAE-specific marker protein that could promote interplay between FAE and immune cells in mucosa-associated lymphoid tissues.

    Original languageEnglish
    Pages (from-to)115-126
    Number of pages12
    JournalCell Structure and Function
    Volume32
    Issue number2
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    Epithelium
    Glycoproteins
    Peyer's Patches
    Pregnancy
    Lymphoid Tissue
    Antigen-Presenting Cells
    Computational Biology
    Interleukin-10
    Placenta
    In Situ Hybridization
    Extracellular Matrix
    Allografts
    Immune System
    Immunity
    Mucous Membrane
    Proteins
    Anti-Inflammatory Agents
    Mothers
    Databases
    Cytokines

    Keywords

    • FAE
    • Microarray
    • Mucosal immunity
    • Peyer's patch
    • Pregnancy-specific glycoprotein 18

    ASJC Scopus subject areas

    • Physiology
    • Structural Biology
    • Cell Biology

    Cite this

    Kawano, K., Ebisawa, M., Hase, K., Fukuda, S., Hijikata, A., Kawano, S., ... Ohno, H. (2007). Psg18 is specifically expressed in follicle-associated epithelium. Cell Structure and Function, 32(2), 115-126. https://doi.org/10.1247/csf.07014

    Psg18 is specifically expressed in follicle-associated epithelium. / Kawano, Kazuya; Ebisawa, Masashi; Hase, Koji; Fukuda, Shinji; Hijikata, Atsushi; Kawano, Sayaka; Date, Yasuhiro; Tsuneda, Satoshi; Itoh, Kikuji; Ohno, Hiroshi.

    In: Cell Structure and Function, Vol. 32, No. 2, 2007, p. 115-126.

    Research output: Contribution to journalArticle

    Kawano, K, Ebisawa, M, Hase, K, Fukuda, S, Hijikata, A, Kawano, S, Date, Y, Tsuneda, S, Itoh, K & Ohno, H 2007, 'Psg18 is specifically expressed in follicle-associated epithelium', Cell Structure and Function, vol. 32, no. 2, pp. 115-126. https://doi.org/10.1247/csf.07014
    Kawano K, Ebisawa M, Hase K, Fukuda S, Hijikata A, Kawano S et al. Psg18 is specifically expressed in follicle-associated epithelium. Cell Structure and Function. 2007;32(2):115-126. https://doi.org/10.1247/csf.07014
    Kawano, Kazuya ; Ebisawa, Masashi ; Hase, Koji ; Fukuda, Shinji ; Hijikata, Atsushi ; Kawano, Sayaka ; Date, Yasuhiro ; Tsuneda, Satoshi ; Itoh, Kikuji ; Ohno, Hiroshi. / Psg18 is specifically expressed in follicle-associated epithelium. In: Cell Structure and Function. 2007 ; Vol. 32, No. 2. pp. 115-126.
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