Psoralen-Containing Vinyl Monomer for Conjugation of Double-Helical DNA with Vinyl Polymers

Mizuo Maeda, Chitoshi Nishimura, Daisuke Umeno, Makoto Takagi

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

We have synthesized a vinyl monomer having a psoralen moiety, which can form a photoadduct with double-helical DNA. The monomer 1 was proved to have an ability to crosslink DNA double strands through a photochemical reaction when irradiated by UV light. The resulting DNA having vinyl groups was copolymerized with a comonomer such as acrylamide and N-isopropylacrylamide to give rise to a DNA—vinyl polymer conjugate. A conjugation based on covalent bondings was verified by using gel electrophoresis; the conjugation efficiency was found to be dependent upon concentration of the monomer which had been used in the antecedent photochemical reaction. This monomer will be a useful tool when anchoring double-helical DNA on polymeric materials for separating and sensing DNA-binding substances.

Original languageEnglish
Pages (from-to)527-531
Number of pages5
JournalBioconjugate Chemistry
Volume5
Issue number6
DOIs
Publication statusPublished - 1994 Nov 1
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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