Pulsatile tubular cardiac tissues fabricated by wrapping human iPS cells-derived cardiomyocyte sheets

Shinpei Tsuruyama, Katsuhisa Matsuura, Katsuhisa Sakaguchi, Tatsuya Shimizu

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to fabricate pulsatile tubular cardiac tissue using cell sheet based-tissue engineering. First, we fabricated human induced pluripotent stem cell (hiPSc)-derived cardiomyocyte sheets and normal human dermal fibroblast (NHDF) sheets which are harvested from temperature responsive culture dishes only by lowering the temperature. Then tubular cardiac tissues are formed by wrapping one hiPSc-derived cardiomyocyte sheet and three NHDF sheets around an octagonal column, and both ends of the tubular tissue were covered with fibrin and collagen gel. The octagonal column with the tubular tissue was connected to an in vitro circulation system in a culture box. After four-day culture, the cardiac tissue survived and pulsated spontaneously in the circulation system. Furthermore, the analysis with a Millar catheter inserted into the cardiac tubes revealed significant inner pressure changes generated by their beating. In addition, the tubular cardiac tissue pulsated in response to the electrical stimulation. Although histological analyses demonstrated that cardiac troponin T-positive cells stratified the inner surface of the tubular tissues, gene expression analyses showed an immature state of these cardiomyocytes. Thus, cell sheet-based tissue engineering realized human pulsatile tubular cardiac tissue fabrication and we believe that these tubular cardiac tissues should contribute to future drug screening and regenerative therapy for heart diseases.

Original languageEnglish
Pages (from-to)297-305
Number of pages9
JournalRegenerative Therapy
Volume11
DOIs
Publication statusPublished - 2019 Dec

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Cardiac Myocytes
Tissue
Induced Pluripotent Stem Cells
Fibroblasts
Tissue Engineering
Stem cells
Tissue engineering
Skin
Troponin T
Preclinical Drug Evaluations
Temperature
Catheters
Fibrin
Collagen
Gene expression
Electric Stimulation
Heart Diseases
Screening
Gels
Cells

Keywords

  • Cardiomyocyte
  • Cell sheet
  • Human induced pluripotent stem cell
  • Regenerative medicine
  • Three-dimensional tissue model
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Developmental Biology

Cite this

Pulsatile tubular cardiac tissues fabricated by wrapping human iPS cells-derived cardiomyocyte sheets. / Tsuruyama, Shinpei; Matsuura, Katsuhisa; Sakaguchi, Katsuhisa; Shimizu, Tatsuya.

In: Regenerative Therapy, Vol. 11, 12.2019, p. 297-305.

Research output: Contribution to journalArticle

Tsuruyama, Shinpei ; Matsuura, Katsuhisa ; Sakaguchi, Katsuhisa ; Shimizu, Tatsuya. / Pulsatile tubular cardiac tissues fabricated by wrapping human iPS cells-derived cardiomyocyte sheets. In: Regenerative Therapy. 2019 ; Vol. 11. pp. 297-305.
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