Purification and characterization of a possible protooncogene fjln product, P59fyn, from a rat brain particulate fraction

Takahiro Miyauchi, Masahiro Ariki, Hirofumi Usui, Kentaro Senba, Yumiko Matsuzawa, Tadashi Yamamoto, Kumao Toyoshima, Masao Takeda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Four tyrosine-protein kinases that reacted with antibodies specific to p62c-yes, p60c-src, p60c-src, and p59fyn, respectively, were solubilized from a rat brain particulate fraction and separated by casein-Toyopearl column chromatography. Possible p59fyn, with a pI of 6.5, was purified 490-fold as a single 69-kDa protein band on SDS-PAGE. The purified enzyme contained almost no phosphotyrosine residues but was autophosphorylated with Mg2+-·ATP exclusively at tyrosine residues, with a concomitant increase in the kinase activity toward tyrosine-glutamate (1 : 4) copolymers. The rate of the copolymer phosphorylation was proportional to the square of the enzyme concentration, suggesting activation through intermolecular catalysis. In the presence of Mn2+, however, the reaction showed a firstorder dependence on the enzyme concentration.

Original languageEnglish
Pages (from-to)729-732
Number of pages4
JournalJournal of Biochemistry
Volume112
Issue number6
Publication statusPublished - 1992 Dec
Externally publishedYes

Fingerprint

Purification
Rats
Brain
Enzymes
Copolymer
Tyrosine
Copolymers
Proteins
Casein
Protein Kinase
Column chromatography
Phosphotyrosine
Phosphorylation
Catalysis
Adenosinetriphosphate
Caseins
Chromatography
Antibody
Antibodies
Protein-Tyrosine Kinases

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Miyauchi, T., Ariki, M., Usui, H., Senba, K., Matsuzawa, Y., Yamamoto, T., ... Takeda, M. (1992). Purification and characterization of a possible protooncogene fjln product, P59fyn, from a rat brain particulate fraction. Journal of Biochemistry, 112(6), 729-732.

Purification and characterization of a possible protooncogene fjln product, P59fyn, from a rat brain particulate fraction. / Miyauchi, Takahiro; Ariki, Masahiro; Usui, Hirofumi; Senba, Kentaro; Matsuzawa, Yumiko; Yamamoto, Tadashi; Toyoshima, Kumao; Takeda, Masao.

In: Journal of Biochemistry, Vol. 112, No. 6, 12.1992, p. 729-732.

Research output: Contribution to journalArticle

Miyauchi, T, Ariki, M, Usui, H, Senba, K, Matsuzawa, Y, Yamamoto, T, Toyoshima, K & Takeda, M 1992, 'Purification and characterization of a possible protooncogene fjln product, P59fyn, from a rat brain particulate fraction', Journal of Biochemistry, vol. 112, no. 6, pp. 729-732.
Miyauchi, Takahiro ; Ariki, Masahiro ; Usui, Hirofumi ; Senba, Kentaro ; Matsuzawa, Yumiko ; Yamamoto, Tadashi ; Toyoshima, Kumao ; Takeda, Masao. / Purification and characterization of a possible protooncogene fjln product, P59fyn, from a rat brain particulate fraction. In: Journal of Biochemistry. 1992 ; Vol. 112, No. 6. pp. 729-732.
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