Purification and characterization of toad prolactin

Kazutoshi Yamamoto, Tetsuya Kobayashi, Sakaé Kikuyama

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Prolactin (PRL) was purified from anterior pituitary glands of adult toad (Bufo japonicus formosus) by extraction of acetone-dried pituitary powder with acid acetone and chromatography on DEAE-cellulose and Sephadex G-100. Purification was monitored by radioimmunoassay developed for bullfrog PRL. Toad PRL thus obtained is as potent as bovine PRL in promoting collagen synthesis in the bullfrog tadpole tail fin. HPLC of the toad PRL on a reverse-phase column shows that the preparation behaves as a homogeneous protein. The hormone has a molecular weight of 23,000 as determined by SDS gel electrophoresis. The isoelectric point is 5.5 as determined by analytical electrofocusing. The amino acid composition closely resembles that of bullfrog PRL.

    Original languageEnglish
    Pages (from-to)104-109
    Number of pages6
    JournalGeneral and Comparative Endocrinology
    Volume63
    Issue number1
    DOIs
    Publication statusPublished - 1986

      Fingerprint

    ASJC Scopus subject areas

    • Endocrinology

    Cite this