Purification and localization of a calcium-binding protein in human spermatozoa

Masahisa Nakamura, Hiromichi Ishikawa, Kouhei Matsuda, Teruaki Iwamoto, Seiji Furuya, Tomoyo Yamanobe, Masayuki Komukai, Kazutoshi Yamamoto, Akihiro Tamura, Shoichi Okinaga, Kiyoshi Arai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The purpose of this study was to purify a calcium-binding protein (CalBP) from human spermatozoa of 226 men and to determine the localization of this protein in spermatozoa. The sperm cells were extracted with 0.6 MKCI, and the KCI extract was then subjected to gel filtration and high performance liquid chromatography. Two CalBPs of Mr = 38 kDa and 52 kDa were found by 45Ca2+-binding on blotted proteins. Only a 52 kDa CalBP (CalBP-52) was purified to a final yield of 0.007%. The isoelectric point of this protein was 5.1. The human CalBP-52 reacted with rabbit antiserum directed against rat 52 kDa CalBP. An immuno-cytochemical study showed that this protein was localized on the sperm head. It is postulated that this protein may have important functions related to Ca2+-transport into sperm cells.

Original languageEnglish
Pages (from-to)500-505
Number of pages6
JournalHuman Reproduction
Volume7
Issue number4
Publication statusPublished - 1992 Apr
Externally publishedYes

Fingerprint

Human Spermatozoa
Calcium-Binding Proteins
Purification
Calcium
Spermatozoa
Proteins
Protein
Sperm Transport
Sperm Head
Isoelectric Point
High performance liquid chromatography
Protein Binding
Gel Chromatography
Rats
Immune Sera
Gels
High Pressure Liquid Chromatography
Carrier Proteins
Localization
Rabbits

Keywords

  • CalBP-52
  • Human
  • Spermatozoa

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Rehabilitation
  • Developmental Biology
  • Physiology

Cite this

Nakamura, M., Ishikawa, H., Matsuda, K., Iwamoto, T., Furuya, S., Yamanobe, T., ... Arai, K. (1992). Purification and localization of a calcium-binding protein in human spermatozoa. Human Reproduction, 7(4), 500-505.

Purification and localization of a calcium-binding protein in human spermatozoa. / Nakamura, Masahisa; Ishikawa, Hiromichi; Matsuda, Kouhei; Iwamoto, Teruaki; Furuya, Seiji; Yamanobe, Tomoyo; Komukai, Masayuki; Yamamoto, Kazutoshi; Tamura, Akihiro; Okinaga, Shoichi; Arai, Kiyoshi.

In: Human Reproduction, Vol. 7, No. 4, 04.1992, p. 500-505.

Research output: Contribution to journalArticle

Nakamura, M, Ishikawa, H, Matsuda, K, Iwamoto, T, Furuya, S, Yamanobe, T, Komukai, M, Yamamoto, K, Tamura, A, Okinaga, S & Arai, K 1992, 'Purification and localization of a calcium-binding protein in human spermatozoa', Human Reproduction, vol. 7, no. 4, pp. 500-505.
Nakamura M, Ishikawa H, Matsuda K, Iwamoto T, Furuya S, Yamanobe T et al. Purification and localization of a calcium-binding protein in human spermatozoa. Human Reproduction. 1992 Apr;7(4):500-505.
Nakamura, Masahisa ; Ishikawa, Hiromichi ; Matsuda, Kouhei ; Iwamoto, Teruaki ; Furuya, Seiji ; Yamanobe, Tomoyo ; Komukai, Masayuki ; Yamamoto, Kazutoshi ; Tamura, Akihiro ; Okinaga, Shoichi ; Arai, Kiyoshi. / Purification and localization of a calcium-binding protein in human spermatozoa. In: Human Reproduction. 1992 ; Vol. 7, No. 4. pp. 500-505.
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