Purification and localization of a calcium-binding protein in human spermatozoa

Masahisa Nakamura, Hiromichi Ishikawa, Kouhei Matsuda, Teruaki Iwamoto, Seiji Furuya, Tomoyo Yamanobe, Masayuki Komukai, Kazutoshi Yamamoto, Akihiro Tamura, Shoichi Okinaga, Kiyoshi Arai

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The purpose of this study was to purify a calcium-binding protein (CalBP) from human spermatozoa of 226 men and to determine the localization of this protein in spermatozoa. The sperm cells were extracted with 0.6 MKCI, and the KCI extract was then subjected to gel filtration and high performance liquid chromatography. Two CalBPs of Mr = 38 kDa and 52 kDa were found by 45Ca2+-binding on blotted proteins. Only a 52 kDa CalBP (CalBP-52) was purified to a final yield of 0.007%. The isoelectric point of this protein was 5.1. The human CalBP-52 reacted with rabbit antiserum directed against rat 52 kDa CalBP. An immuno-cytochemical study showed that this protein was localized on the sperm head. It is postulated that this protein may have important functions related to Ca2+-transport into sperm cells.

Original languageEnglish
Pages (from-to)500-505
Number of pages6
JournalHuman Reproduction
Volume7
Issue number4
Publication statusPublished - 1992 Apr
Externally publishedYes

Keywords

  • CalBP-52
  • Human
  • Spermatozoa

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Rehabilitation
  • Developmental Biology
  • Physiology

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