Purification and Some Properties of an Alkaline Pullulanase from Alkalophilic Bacillus sp. KSM-1876

Katsutoshi Ara, Kazuaki Igarashi, Katsuhisa Saeki, Shuji Kawai, Susumu Ito

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A novel alkaline pullulanase (pullulan 6-glucanohydrolase, EC 3.2.1.41) was purified to homogeneity from the culture filtrate of the alkalophilic Bacillus sp. KSM-1876. The pullulanase had an optimum pH for activity of around 10.0-10.5, which is the highest pH for optimum activity of any pullulanases reported to date. The optimum temperature at pH 10 was around 50°C. The enzyme had a molecular mass of 120 kDa and an isoelectric point of pH 5.2. The enzyme acted specifically on pullulan to generate maltotriose as the major end product; the Km for pullulan was 1.8mg/ml. N-Bromosuccinimide abolished the enzymatic activity, and pullulan protected the enzyme from inactivation by this tryptophan-specific oxidant, suggesting that a tryptophan residue(s) is involved in the mechanism of action of the pullulanase from Bacillus sp. KSM-1876.

Original languageEnglish
Pages (from-to)62-65
Number of pages4
JournalBioscience, biotechnology, and biochemistry
Volume56
Issue number1
DOIs
Publication statusPublished - 1992 Jan 1
Externally publishedYes

Fingerprint

Bacilli
Bacillus
Purification
Tryptophan
Enzymes
Bromosuccinimide
Molecular mass
Oxidants
Isoelectric Point
Temperature
pullulanase
pullulan

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Purification and Some Properties of an Alkaline Pullulanase from Alkalophilic Bacillus sp. KSM-1876. / Ara, Katsutoshi; Igarashi, Kazuaki; Saeki, Katsuhisa; Kawai, Shuji; Ito, Susumu.

In: Bioscience, biotechnology, and biochemistry, Vol. 56, No. 1, 01.01.1992, p. 62-65.

Research output: Contribution to journalArticle

Ara, Katsutoshi ; Igarashi, Kazuaki ; Saeki, Katsuhisa ; Kawai, Shuji ; Ito, Susumu. / Purification and Some Properties of an Alkaline Pullulanase from Alkalophilic Bacillus sp. KSM-1876. In: Bioscience, biotechnology, and biochemistry. 1992 ; Vol. 56, No. 1. pp. 62-65.
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